贵州医科大学学报

2014, v.39;No.168(03) 302-306

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登革2型病毒及其NGC株、M株E基因的原核蛋白对HUVEC通透性的影响
The Effect on HUVEC Permeability by Dengue Virus Type 2 and Prokaryotic Expression of E Gene of Two Strains

任玮;左丽;吕秉乐;
REN Wei;ZUO Li;LV Bingle;Department of Immunology,Guiyang Medical College;

摘要(Abstract):

目的:研究登革2型病毒(DENV-2)、登革2型病毒国际参考株(NGC)E基因1-476序列原核蛋白、登革2型病毒贵州蚊虫分离株(M)E基因1-476序列原核蛋白对人脐静脉内皮细胞(HUVEC)通透性的影响。方法:将DENV-2 M株和NGC株E基因1-476序列原核表达产物及DENV-2接种于HUVEC单层细胞,用transwell法检测HUVEC的通透性,透射电镜观察细胞超微结构的改变。结果:DENV-2、NGC株E基因的原核蛋白、M株E基因的原核蛋白分别在30 min、3 h、12 h时对HUVEC通透性的影响最为明显,透射电镜结果显示有细胞超微结构的改变。结论:DENV-2、DENV-2 NGC株E基因原核蛋白、M株E基因原核蛋白对HUVEC通透性、细胞超微结构有明显影响。
Objective: To study the effect on human umbilical vein endothelial cell(HUVEC) permeability by Dengue Virus type 2(DENV-2)and prokaryotic protein of 1- 476 sequences of dengue virus type 2 M and NGC strains. Methods: HUVEC monolayer cells were vaccinated with E gene 1-476 sequence prokaryotic expression products of strains DENV-2 M and DENV-2 NGC,as well as DENV-2. The HUVEC permeability was tested by Transwell method,and transmission electron microscope(TEM) was used to observe ultrastructure changes of cells. Results: Effects on HUVEC permeability of DENV-2,NGC strain E gene prokaryotic protein and M strain E gene prokaryotic protein were most obvious at 30 minutes,3 hours,and 12 hours of the experiment respectively. TEM results presented ultrastructure changes of cells. Conclusion: HUVEC and HUVEC permeability can be obviously influenced by DENV-2,and E gene prokaryotic proteins of DENV-2 NGC strain and M strain.

关键词(KeyWords): 登革热病毒;E基因;内皮,血管;脐静脉;细胞膜通透性
dengue virus;E gene;endothelium,vascular;umbilical veins;cell membrane permeability

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(30560148);; 国家973前期研究重点项目(2008CB517408)

作者(Author): 任玮;左丽;吕秉乐;
REN Wei;ZUO Li;LV Bingle;Department of Immunology,Guiyang Medical College;

Email:

DOI: 10.19367/j.cnki.1000-2707.2014.03.006

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