席晓岚,徐红,徐科,季宇飞,刘红
XI Xiao-lan~1, XU Hong~1, XU Ke~1, JI Yu-fei~2, LIU Hong~1 (1.Department of Chemistry, Guiyang Medical College, Guiyang 550004, China; 2. Guiyang Environmental Monitoring Central Station, Guiyang 550002, China)

• 1:贵阳医学院化学教研室
• 2:贵阳医学院化学教研室
• 3:贵阳医学院化学教研室
• 4:贵阳市环境监测中心站

摘要(Abstract)：

目的:研究亚硝酸根与甲基蓝的褪色反应,建立一个简单、灵敏、选择性高的亚硝酸根的测定方法。方法:采用紫外分光光度法测定不同条件下的吸光度。结果:实验表明,当甲基蓝与NO2-反应后发生颜色变化,在590 nm波长处发生褪色反应,此长波处,吸光度的变化与溶液中NO2-的浓度成正比,其线性范围在0⁵ m g/L,摩尔吸光系数为ε=1.4×104L.m ol-1.cm-1,相关系数为0.995 5。结论:甲基蓝分光光度法具有良好的稳定性和选择性,用于环境水样中微量NO2-的测定,结果满意;建立了测定亚硝酸根的新方法。
Objective: To explore a new spectrophotometric method for the determination of trace nitrite. Method: Based on the nitrosation reaction of methyl blue with nitrite in diluted hydrochloric acid medium, a new method was developed. Results: The linear range for the determination was 0～50mg/L NO_25 m g/L,摩尔吸光系数为ε=1.4×104L.m ol-1.cm-1,相关系数为0.995 5。结论:甲基蓝分光光度法具有良好的稳定性和选择性,用于环境水样中微量NO2-的测定,结果满意;建立了测定亚硝酸根的新方法。
Objective: To explore a new spectrophotometric method for the determination of trace nitrite. Method: Based on the nitrosation reaction of methyl blue with nitrite in diluted hydrochloric acid medium, a new method was developed. Results: The linear range for the determination was 0～50mg/L NO_2^- . Most of coexistent ions did not interfere the determination of NO_2- . Most of coexistent ions did not interfere the determination of NO_2^-. When applied this method to the determination of nitrite in water samples, satisfactory results were achieved. Conclusion: The development of this method is successful.

关键词(KeyWords)： 亚硝酸盐类;分光光度法,紫外线;甲基蓝
nitrites; spectrophotometry,ultraviolet; methyl blue

Abstract:

Keywords:

基金项目(Foundation): 市科技局基金资助项目(T-2004-4)

作者(Authors): 席晓岚,徐红,徐科,季宇飞,刘红
XI Xiao-lan~1, XU Hong~1, XU Ke~1, JI Yu-fei~2, LIU Hong~1 (1.Department of Chemistry, Guiyang Medical College, Guiyang 550004, China; 2. Guiyang Environmental Monitoring Central Station, Guiyang 550002, China)

DOI: 10.19367/j.cnki.1000-2707.2005.04.016

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