贵州医科大学学报

2014, v.39;No.166(01) 5-7+17

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人脐带间充质干细胞过敏性试验
Allergy Test of Human Umbilical Cord Mesenchymal Stem Cells

许键炜;何志旭;舒莉萍;王凤昌;
XU Jianwei;HE Zhixu;SHU Liping;WANG Fengchang;Stem Cell and Tissue Engineering Research Center,Guiyang Medical College;Department of Pediatrics,the Affiliated Hospital of Guiyang Medical College;Department of Immunology,Guiyang Medical College;The Center of Animal Laboratory ,Guiyang Medical College;

摘要(Abstract):

目的:观察人脐带间充质干细胞对豚鼠的过敏性反应,为临床使用人脐带间充质干细胞的安全性提供参考。方法:培养扩增人脐带来源的间充质干细胞,制备成细胞生理盐水混悬液。将30只豚鼠分成3组,分别是模型1组、模型2组和对照组。2个模型组隔日肌注细胞生理盐水混悬液3次,实验1组于首次注射后第14天,实验2组于首次注射后第21天分别静脉注射混悬液进行攻击。观察动物有无过敏反应症状。结果:动物无明显过敏反应症状,反应级数为0级。结论:人脐带间充质干细胞免疫原性低,不易导致过敏反应。
Objective: To observe allergic reactions of guinea pigs caused by human umbilical cord mesenchymal stem cells( HUCMSCs),and so as to provide reference for the security of clinical use of HUCMSCs. Methods: HUCMSCs were cultured and amplified,and prepared into cell and normal saline suspension. Tirty guinea pigs were divided into 3 groups: the first model group,the second model group and control group. Guinea pigs in the two model groups were injected with the suspension every other day for three times,in the 14thdays and the 21thdays after the first injection,guinea pigs in the first and second model groups were intravenously injected with the suspension again respectively. whether allergic reaction occured were observed. Results: The animals had no obvious allergic symptoms,the reaction order was 0. Conclusions: HUCMSCs have low immunogenicity and are uneasy to cause allergic reactions.

关键词(KeyWords): 豚鼠;干细胞;过敏反应;细胞培养;人脐带间充质干细胞
guinea pigs;stem cells;anaphylaxis;cell culture;human umbilical cord mesenchymal stem cells

Abstract:

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基金项目(Foundation): 国家自然科学基金资助项目(No.30560159);; 贵州省“125计划”重大科技专项(黔教合重大专项字[2013]020);; 贵州省教育厅自然科学研究项目(黔教合KY字[2012]036号)

作者(Author): 许键炜;何志旭;舒莉萍;王凤昌;
XU Jianwei;HE Zhixu;SHU Liping;WANG Fengchang;Stem Cell and Tissue Engineering Research Center,Guiyang Medical College;Department of Pediatrics,the Affiliated Hospital of Guiyang Medical College;Department of Immunology,Guiyang Medical College;The Center of Animal Laboratory ,Guiyang Medical College;

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DOI: 10.19367/j.cnki.1000-2707.2014.01.004

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