D-松醇原药对大鼠肝脏的毒性作用D-pinitol Technical Grade Toxicity on Rats Liver
曾奇兵,徐玉艳,张婷,唐艳明,李敏,洪峰,王荣
ZENG Qibing,XU Yuyan,ZHANG Ting,TANG Yanming,LI Min,HONG Feng,WANG Rong
摘要(Abstract)：
目的:探讨D-松醇原药对大鼠肝脏的毒性。方法:4~6周龄健康清洁级SD大鼠80只(雌雄各半),随机分成D-松醇原药低剂量组、中剂量组、高剂量组及对照组,每组20只,雌雄各10只,低、中、高剂量组分别喂饲390.63 mg/kg、1 562.50 mg/kg和6 250.00 mg/kg的D-松醇原药饲料,对照组喂饲普通标准饲料,连续喂饲90 d;观察4组大鼠的一般情况、体重变化;第90天时,各组大鼠心脏取血检测天冬氨酸氨基转换酶(AST)、丙氨酸氨基转换酶(ALT)、碱性磷酸酶(ALP)、总蛋白(TP)、白蛋白(ALB)、球蛋白(GLO)、血糖(GLU)、总胆固醇(CHO)、总胆红素(TBLL)、胆碱酯酶(CHE)10项肝功能指标,取各组大鼠肝脏计算肝脏脏体比及观察肝脏病理组织学改变。结果:D-松醇原药低、中、高剂量组雄性大鼠实际染毒量分别为33.58 mg/kg、149.39 mg/kg、543.98 mg/kg;雌性大鼠实际染毒剂量分别为30.88 mg/kg、127.06 mg/kg、483.88 mg/kg。高剂量组雌性大鼠GLO、TP低于对照组(P<0.05);中、高剂量组雌性大鼠CHE高于对照组(P<0.05);中、高剂量组雄性大鼠TBLL高于对照组(P<0.05)。结论:D-松醇原药90 d经口喂养最大无作用剂量,雄性为30.88 mg/kg、雌性为33.58 mg/kg。雄性大鼠摄入D-松醇原药127.06 mg/kg以及雌性大鼠摄入D-松醇原药149.39 mg/kg时可以引起大鼠肝脏损伤。
Objective: To investigate D-pinitol technical grade toxicity on rats liver. Methods:Eighty healthy SD rats of 4 to 6 weeks of age( equal male and female number),randomly divided into D-pinitol technical grade low,medium and high dose groups and control group,with 10 male and 10 female rats each; low,medium and high dose groups were fed with 390. 63 mg / kg,1 562. 50 mg / kg and 6 250. 00 mg / kg D-pinitol technical grade,control group was fed with normal standard stuff,continuous fed for 90 days; observing general condition of 4 groups and weight change; on 90 th day,heart blood testing on liver function index of AST,ALT,ALP,TP,ALB,GLO,GLU,CHO,TBLL and CHE. Extracting rats' liver to calculate liver ratio and liver pathological changes. Results: The actual intoxicated amount of male rats in low,middle and high dose group were 33. 58 mg / kg,149. 39 mg / kg and 543. 98 mg / kg; and the actual intoxicated amount of female rats were 30. 88 mg / kg,127. 06 mg /kg,483. 88 mg / kg. Globulin( GLO),total protein( TP) of female rats in high dose group was less than that of control group( P < 0. 05). Cholinesterase( CHE) of female rats in middle and high dose group was higher than that of control group( P < 0. 05). Conclusion: D-pinitol technical grade fed for90 days no-effect levels were as follows: 127. 06 mg / kg for male rats and 149. 39 mg / kg for femal rats of D-pinitol technical grade can would cause liver damage in rats.
关键词(KeyWords)：
药物毒性;农药;D-松醇;大鼠,Sprague-Danley;肝脏
drug toxicity;pesticide;D-pinitol;rats;Sprague-Danley;liver
基金项目(Foundation):
作者(Author):
曾奇兵,徐玉艳,张婷,唐艳明,李敏,洪峰,王荣
ZENG Qibing,XU Yuyan,ZHANG Ting,TANG Yanming,LI Min,HONG Feng,WANG Rong
DOI: 10.19367/j.cnki.1000-2707.2016.02.018
参考文献(References)：
- [1]胡云平,周志俊,孙运光,等.乐果对培养乳鼠心肌细胞的毒作用[J].卫生毒理学杂志,2000(4):209-211.
- [2]徐玉艳,曾奇兵,洪峰.95%氨氯吡啶酸对大鼠肝脏的损害作用[J].湖北农业科学,2013(9):2041-2043.
- [3]王丹丹,陈佳,陈义娟,等.D-松醇原药及其水剂对黄瓜白粉病的作用方式[J].农药,2012(4):292-295.
- [4]Chaubal R,Pawar PV,Hebbalkar GD,et al.Larvicidal activity of Acacia nilotica extracts and isolation of D-pinitol-a bioactive carbohydrate[J].Chem Biodivers,2005(5):684-688.
- [5]国家技术监督局.GB 15670-1995中国标准书号[S].北京:中国标准出版社,1995.
- [6]何国平,贾菠,郭昌烜,等.90 d喂养氟磺唑草胺原药对大鼠毒性作用的研究[J].毒理学杂志,2013(4):277-279.
- [7]刘树业,段樱,李娴.代谢组学的进展及肝脏代谢组学[J].分子诊断与治疗杂志,2009(3):197-202.
- [8]马晓明,宋祥福,薛茗方,等.碘甲烷对大鼠肝脏合成功能的影响[J].中国比较医学杂志,2013(9):19-22.
- [9]陈文彬,潘祥林.诊断学[M].7版.北京:人民卫生出版社,2008:363-365.
文章评论(Comment)：
|
||||||||||||||||||
|
||||||||||||||||||