江明礼,莫非,渠巍,孙朝琴,罗昭逊,张姝,张梦薇,吴琼
JIANG Mingli,MO Fei,QU Wei,SUN Chaoqin,LUO Zhaoxun,ZHANG Shu,ZHANG Mengwei,WU Qiong

• 1:贵州医科大学
• 2:贵阳市妇幼保健院检验科

摘要(Abstract)：

目的:探讨苗药头花蓼对幽门螺杆菌相关性胃炎(HAG)SD大鼠胃黏膜组织中PTEN、PI3K和AKT表达的影响。方法:48只SPF级SD大鼠分为空白组、模型组及药物组,模型组和药物组SD大鼠采用H.pylori悉尼驯化株SS2000灌胃构建H.pylori感染慢性胃炎动物模型;建模成功后,药物组给予1.58 g/(kg·d)头花蓼生药2周,空白组和模型组给予等量无菌生理盐水;末次给药4周后,处死各组大鼠取胃组织,采用HE染色观察胃黏膜组织变化,并进行病理评分;提取各组大鼠胃黏膜组织蛋白及mRNA,采用Western Blot和Real time PCR技术检测大鼠胃黏膜组织中PTEN、PI3K和AKT蛋白及mRNA表达量的变化。结果:HE染色结果显示,模型组SD大鼠胃黏膜呈现慢性炎症,病理评分明显升高(P<0.05),头花蓼药物组病理评分较模型组显著降低(P<0.05);Western blot及Real time PCR结果显示,模型组PTEN蛋白及mRNA表达较空白组明显降低(P<0.05),AKT表达显著升高(P<0.05);与模型组比较,药物组胃黏膜中PTEN蛋白及mRNA表达上调(P<0.05),AKT表达水平下调(P<0.05)。结论:苗药头花蓼可能通过上调PTEN表达、抑制AKT/PI3K通路活化,从而改善H.pylori引起的胃黏膜炎症。
Objective: To investigate the effect of Polygonum capitatum on the expression of PTENN,PI3K and AKT in gastric mucosa of rats with Helicobacter pylori. Methods: Forty-eight SPF SD rats were divided into blank group,model group and drug group,and SD rats in model and drug groups were given H. pylori strain SS2000 to establish H. pylori infected chronic gastritis animal model. After the model was successfully established,the drug group was given 1. 58 g crude drug/kg · d for 2 weeks,and the blank group and the model group were given the same amount of aseptic saline. Four weeks after the last administration,the rats in each group were killed to take gastric tissue,the changes of gastric mucosal tissue were observed by HE staining,and the pathological score was evaluated.Gastric mucosal protein and mRNAs were extracted from rats in each group. Western blot and Real time polymerase chain reaction were used to detect the changes of PTENN,PI3K,AKT protein and mRNA expression in gastric mucosa of rats. Results: The results of HE staining showed that chronic inflammation and the pathological score of gastric mucosa in SD rats of model group were significantly increased( P < 0. 05),and the pathological score in the drug group of Polygonum capitatum was significantly lower than that in the model group( P < 0. 05). The results of Western blot and Real time PCR showed that the expression of PTEN protein and mRNA in the model group was significantly lower than that in the blank group( P < 0. 05),and AKT expression increased significantly( P < 0. 05). Compared with the model group,the expression of PTEN protein and mRNA in gastric mucosa of drug group was up-regulated( P < 0. 05),and the level of AKT expression was down-regulated( P < 0. 05).Conclusion: Polygonum capitatum can inhibit the activation of AKT/PI3K pathway by upregulating PTEN expression,thus gastric mucosal inflammation induced by H. pylori can be improved.

关键词(KeyWords)： 苗药;头花蓼;幽门螺杆菌;胃炎;信号转导
Miao medicine;Polygonum capitatum;Helicobacter pylori;gastritis;signal transduction

Abstract:

Keywords:

基金项目(Foundation): 贵州省科技合作计划基金[黔科合LH(2015)7417号];; 贵州省卫生计生委科学技术基金(gzwjkj2015-1-003);; 贵阳市科技计划项目[筑科合同(20141001)号];; 贵州省中医药管理局中医药、民族医药科学技术研究课题(QZYY-2016-005)

作者(Author): 江明礼,莫非,渠巍,孙朝琴,罗昭逊,张姝,张梦薇,吴琼
JIANG Mingli,MO Fei,QU Wei,SUN Chaoqin,LUO Zhaoxun,ZHANG Shu,ZHANG Mengwei,WU Qiong

DOI: 10.19367/j.cnki.1000-2707.2018.08.004

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