高原,徐沁,褚孟洋,张丽君,李程飞,石菲,孙喜庆,赵疆东,闫铭,王永春
GAO Yuan,XU Qin,CHU Mengyang,ZHANG Lijun,LI Chengfei,SHI Fei,SUN Xiqing,ZHAO Jiangdong,YAN Ming,WANG Yongchun

• 1:第四军医大学航空航天医学系航空航天生物动力学教研室
• 2:第四军医大学学员一旅
• 3:第四军医大学西京医院骨科

摘要(Abstract)：

目的:检测模拟失重48 h人肺微血管内皮细胞(HMVEC-L)miR-1246和miR-1261表达的改变,利用生物信息学方法分析预测其靶基因。方法:以回转器行模拟失重效应48 h的HMVEC-L为模拟失重组,同时设正常重力对照组,比较两组细胞miRNA表达谱的变化,应用Real-time PCR检测HMVEC-L细胞miR-1246和miR-1261的表达,通过Target Scan、miRDB和miRanda对miR-1246和miR-1261的靶基因进行预测以及功能注释和通路富集分析。结果:与正常重力对照组比较,模拟失重48 h可引起HMVEC-L细胞中29种miRNA呈现显著差异性表达,miR-1246和miR-1261显著降低,差异有统计学意义(P<0.05);生物信息学分析显示miR-1246和miR-1261的靶基因涉及生物过程、分子功能及细胞组成等多种功能,主要参与了磷酸化通路、凋亡相关通路和细胞骨架等相关分子的调控。结论:模拟失重48 h后可引起HMVEC-L细胞miR-1246和miR-1261的表达降低,提示其可能参与了模拟失重后内皮细胞功能异常的调控。
Objective: To detect the changes of miR-1246 and miR-1261 expression after 48 h simulated weightlessness in human pulmonary microvascular endothelial cells,and further explore the predicted target gene by bioinformatics analysis. Methods: The clinostat was employed for simulated weightlessness,HMVEC-L underwent 48 h as simulated weightless group,and normal gravity group was set as control group. Comparing changes of miRNAs,real-time PCR was applied to confirm the expression of miR-1246 and miR-1261 obtained by microarray analysis. The possible target genes of miR-1246 and miR-1261 were predicted,functional annotated,pathway enrichment analyzed by TargetS can,miRDB and miRanda. Results: Compared with the control group,48 h simulated weightlesscould induce 29 miRNAs expressions with significant changes in HMVEC-L( P < 0. 05); the expression of miR-1246 and miR-1261 decreased significantly( P < 0. 05). The predicted miR-1246 and miR-1261 target genes by bioinformatics analysis were involved in biological process,molecular function and cellular composition,which composed the molecular regulation of phosphorylation pathway,apoptosis pathway and cytoskeleton. Conclusion: The expression of miR-1246 and miR-1261 was obviously decreased in HMVEC-L induced by 48 h simulated weightlessness. Bioinformatics analysis showed the changes of miR-1246 and miR-1261 expression might involve in the regulation of dysfunction of endothelial cells induced by simulated weightlessness.

关键词(KeyWords)： 肺;模拟失重;血管内皮细胞;miR-1246;miR-1261;生物信息学
lung;simulated weightlessness;vascular endothelial cells;miR-1246;miR-1261;bioinformatics

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81372130,81301681,81301581,81471817)

作者(Author): 高原,徐沁,褚孟洋,张丽君,李程飞,石菲,孙喜庆,赵疆东,闫铭,王永春
GAO Yuan,XU Qin,CHU Mengyang,ZHANG Lijun,LI Chengfei,SHI Fei,SUN Xiqing,ZHAO Jiangdong,YAN Ming,WANG Yongchun

DOI: 10.19367/j.cnki.1000-2707.2017.06.006

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