刘丰丰,青磊,王海宁,黄作苏
LIU Feng-feng 1; QING Lei 2; WANG Hai-ning 3; HUANG Zuo-su 4 (1. The Affiliated Hospital of Guiyang Medical College;

• 1:贵阳医学院附院
• 2:解放军第四十四医院
• 3:贵州省药品检验所
• 4:中国人民解放军九五五六七部队 贵州贵阳550004

摘要(Abstract)：

目的 :测定阿归养血颗粒中阿魏酸的含量。方法 :用高效液相色谱法 (HPLC)测定阿魏酸的含量。色谱分析采用DiamonsilTM(砖石 )C18色谱分析柱 ,柱温 30℃ ;甲醇 醋酸 (5 0∶5 0 )为流动相 ,紫外检测波长为 313nm ,流速 1 0ml/min ,实验数据用Gilson 715液相色谱工作站处理 ,以峰面积外标法定量。结果 :5种质控样品的平均回收率为 10 0 4 4 % (V/V) ,日内和日间差RSD为 2 0 8%。结论 :本法精度度好 ,方法简便、快速 ,重视性好 ,可以较好地控制阿魏酸的含量标准
Objective: To determine the content of ferulic acid in Aguiyangxue granules. Methods: HPLC was used to determine the ferulic acid content, and the chromatographic separation was performed on a Diamonsil TmC 18 column. The column temperature was 30℃ with methanol-1.0% NH 4AC (50∶50) as mobile phase. The detection wavelength was 313 nm, and the flow rate 1 ml·min -1. The test data were treated with Gilson 715 chro-matographic workstation. Peak area calculated represented the amount. Results: The average recovery was 100.44% for the 5 batches of samples. Conclusions: The method is accurate, simple and sensitive. This method has good recovery rate and reliability. It could be used for controlling the content standard of ferulic acid.

关键词(KeyWords)： 色谱法,高压液相;植物制剂;阿魏酸;阿归养血颗粒
chromatography,high pressure liquid; plant preparation; ferulic acid; Aguiyangxue granules

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 刘丰丰,青磊,王海宁,黄作苏
LIU Feng-feng 1; QING Lei 2; WANG Hai-ning 3; HUANG Zuo-su 4 (1. The Affiliated Hospital of Guiyang Medical College;

DOI: 10.19367/j.cnki.1000-2707.2004.03.016

参考文献(References)：

• [1]苗明山.现代实用中药控制技术与应用[M].北京:化学工业出版社,2001.412

文章评论(Comment)：