罗晓旋,黄芬
LUO Xiaoxuan,HUANG Fen

• 1:汕头潮南民生医院妇产科

摘要(Abstract)：

目的:探讨间叶上皮转化(MET)在子宫平滑肌肿瘤发生发展中的作用及机制。方法:78例子宫平滑肌肿瘤手术切除后的石蜡标本分为子宫平滑肌瘤组(UL组,n=31)、富于细胞性子宫平滑肌瘤组(CUL组,n=32)及子宫平滑肌肉瘤组(LMS组,n=15),选择35例正常子宫肌层石蜡标本作为对照组;采用免疫组织化学染色法检测4组标本中MET相关因子E-钙黏附蛋白(E-cadherin)、β-连环蛋白(β-catenin)及锌指转录因子(Slug)蛋白表达,比较4组标本E-cadherin、β-catenin及Slug的表达阳性率,采用Spearman法分析E-cadherin、β-catenin及Slug表达的相关性,比较不同临床病理分型LMS患者间E-cadherin、β-catenin及Slug的表达差异。结果:与对照组比较,各组子宫平滑肌肿瘤中E-cadherin、β-catenin表达阳性率升高、Slug表达阳性率降低(P <0. 05);与CUL组及UL组比较,LMS组E-cadherin和β-catenin的表达阳性率升高、Slug表达阳性率降低(P <0. 05); CUL组与UL组上述3个指标比较,Slug表达阳性率低于UL组(P <0. 05); Spearman相关性分析发现,E-cadherin与β-catenin表达呈现正相关(r=0. 753,P <0. 05),E-cadherin、β-catenin与Slug呈负相关(r=-0. 795、-0. 703,P <0. 05);在不同临床病理分型的LMS标本中,与分化程度低、有浸润转移及Ⅱ~Ⅳ期比较,细胞分化程度高、无浸润转移及Ⅰ期的LMS标本中E-cadherin、β-catenin表达阳性率明显高、Slug阳性表达率明显降低,差异有统计学意义(P <0. 05)。结论:MET存在于子宫平滑肌肿瘤的发病过程中,其分子标记物E-cadherin、β-catenin及Slug可能参与了分化、浸润转移及病理分期等过程。
Objective: To investigate the role of mesenchymal epithelial transformation in uterine smooth muscle tumors and its pathogenesis. Methods: 78 paraffin specimens of uterine smooth muscle tumors after surgical resection were divided into the uterine leiomyoma(UL) group(n = 31),the cellular uterine leiomyoma(CUL) group(n = 32),and the uterine leiomyosarcoma(LMS) group(n =15). 35 normal myometrium paraffin specimens were chosen as the control group. The expressions of E-cadherin,β-catenin and(Slug) in the four groups of specimens were detected by immunohistochemical staining and analyzed by Spearman method. The expressions of E-cadherin、β-catenin and Slug among LMS patients with different clinical pathological types were also compared. Results: Compared with the control group,the positive rate of E-cadherin and β-catenin in uterine smooth muscle tumors increased and the positive rate of Slug decreased(P < 0. 05). Compared with the CUL group and the UL group,the positive rate of E-cadherin and β-catenin in the LMS group increased while the positive rate of Slug expressions decreased(P < 0. 05). The positive rate of Slug expression in the CUL group was lower than that in the UL group(P < 0. 05). Spearman correlation analysis showed that there was a positive correlation between E-cadherin and β-catenin expression(r = 0. 753,P <0. 05),but a negative correlation between E-cadherin and β-catenin and Slug(r =-0. 795、-0. 703,P < 0. 05). In the LMS specimens of different clinicopathologic types,compared with those of low cell differentiation degree,with infiltration and metastasis,and in stage Ⅱ~ Ⅳ,the positive rate of E-cadherin and β-catenin were significantly higher and the positive rate of Slug was significantly lower in those with high cell differentiation,without infiltration and metastasis,and in stage I. The differences were statistically significant(P < 0. 05). Conclusions: Mesenchymal Epithelial transformation is present in the development of uterine smooth muscle tumors. Its molecular markers such as E-cadherin,β-catenin and Slug may be involved in differentiation,invasion,metastasis and pathological staging.

关键词(KeyWords)： 子宫肿瘤;平滑肌瘤;间叶上皮转化;E-钙黏附蛋白;β-连环蛋白;锌指转录因子
uterine tumor;smooth muscle tumor;mesenchymal epithelium transformation;E-cadherin;β-catenin;zinc finger transcription factor

Abstract:

Keywords:

基金项目(Foundation): 广东省医疗卫生科技计划引导项目(20150406)

作者(Author): 罗晓旋,黄芬
LUO Xiaoxuan,HUANG Fen

DOI: 10.19367/j.cnki.1000-2707.2018.11.024

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