张先俊,胡建国,杨力
ZHANG Xianjun,HU Jianguo,YANG Li(1.Department of Gastroenterology

• 1:潜江市中心医院消化内科
• 2:宁夏医科大学附属医院消化内科

摘要(Abstract)：

目的:探讨乙型肝炎肝硬化患者血清可溶性细胞黏附分子-1(sICAM-1)、白细胞介素-18(IL-18)的变化与乙型肝炎病毒(HBV)复制的关系。方法:采用ELIAS对60例乙型肝炎肝硬化患者和30例健康人血清sICAM-1、IL-18水平进行测定,用荧光定量PCR仪检测60例乙型肝炎肝硬化患者HBV-DNA含量。结果:肝硬化组患者血清sICAM-1、IL-18水平明显高于健康对照组(P<0.01),血清sICAM-1水平与IL-18呈显著正相关(r=0.432,P<0.01);肝硬化患者HBV-DNA阳性组血清sICAM-1水平与HBV-DNA阴性组相比较无显著性差异(P>0.05);肝硬化患者HBV-DNA阳性组血清IL-18水平与HBV-DNA阴性组相比较有显著性差异(P<0.01)。结论:sICAM-1水平变化与HBV的复制水平无关,继发性免疫反应导致sICAM-1表达,sICAM-1造成肝细胞损伤;IL-18水平变化与HBV复制活跃程度相关,测定IL-18活性,在监测HBV病毒复制状态及抗病毒治疗等方面都具有一定的实用价值。
Objective: To investigate the changes of serum soluble intercellular adhesion molecule-1(sICAM-1) and interleukin-18(IL-18) in patients with type B hepatitis cirrhosis,and their relationship with HBV replication.Methods: Serum levels of sICAM-1 and IL-18 of 60 type B hepatitis cirrhosis patients(group A) and 30 normal persons(group B) were detected with enzyme linked immunosorbent assay(ELISA).The serum level of HBV-DNA in patients of group A was detected with fluorescent quantitative PCR.Results: The serum levels of sICAM-1 and IL-18 in group A were significantly higher than those in group B(P<0.01).There was a positive correlation between serum levels of sICAM-1 and IL-18.There was no significant difference in serum levels of sICAM-1 between HBV-DNA positive group and HBV-DNA negative group in group A(P>0.05),but there was significant difference in serum levels of IL-18 between them in group A(P<0.01).Conclusions: The change of serum level of sICAM-1 in patients with type B hepatitis cirrhosis does not relate to replication of HBV.It suggests that secondary immune injury lead to the expression of sICAM-1,which results in injury of liver cells.Since change of IL-18 serum level in these patients relates to replication of HBV,measuring IL-18 level has some practical value in monitoring HBV replication and antiviral therapy application.

关键词(KeyWords)： 肝炎,乙型;肝硬化;细胞黏附分子;白细胞介素-18;DNA复制
Hepatitis B;liver cirrhosis;cell adhesion molecules;interleukin-18;DNA replication

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 张先俊,胡建国,杨力
ZHANG Xianjun,HU Jianguo,YANG Li(1.Department of Gastroenterology

DOI: 10.19367/j.cnki.1000-2707.2010.06.016

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