张禹;严志凌;史荔;杨宏英;杨家方;俞建昆;张新文;刘舒媛;李传印;姚宇峰;
ZHANG Yu;YAN Zhiling;SHI Li;YANG Hongying;YANG Jiafang;YU Jiankun;ZHANG Xinwen;LIU Shuyuan;LI Chuanyin;YAO Yufeng;Institute of Medical Biology,Chinese Academy of Medical Sciences & Peking Union Medical College;Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Disease;The Third Affiliated Hospital of Kunming Medical University;

• 1:中国医学科学院&北京协和医学院医学生物学研究所
• 2:云南省重大传染病疫苗研发重点实验室
• 3:昆明医科大学附属第三医院

摘要(Abstract)：

目的:探讨脂联素基因启动子区单核苷酸多态(SNP)与宫颈癌的相关性。方法:选取云南地区汉族女性人群中宫颈癌患者395例组为癌症组、癌前病变者131例为癌前病变组,正常健康体检者685例作为对照组,采用Taq Man探针基因分型方法对3组人群外周血DNA中脂联素基因启动子区的3个SNP位点rs266730(G>A)、rs266729(C>G)和rs16861194(A>G)进行基因分型,并构建单倍型,评估上述3个SNP位点及单倍型与宫颈癌的相关性。结果:癌症组、癌前病变组及对照组间脂联素基因启动子区的3个SNP位点rs266730(G>A)、rs266729(C>G)和rs16861194(A>G)的基因频率和等位基因频率比较,差异均无统计学意义(P>0.05);rs266730(G>A)、rs266729(C>G)和rs16861194(A>G)构建的单倍型频率在癌症组、癌前病变组和对照组中的差异无统计学意义(P>0.05)。结论:在云南汉族女性群体中,脂联素基因启动子区的rs266730(G>A)、rs266729(C>G)和rs16861194(A>G)3个SNP位点多态性与宫颈癌的发生无关。
Objective: To evaluate the association of single nucleotide polymorphisms( SNPs) in the adiponectin gene promoter region with the occurrence and development of cervical cancer in Yunnan Han population. Methods: 395 patients with cervical cancer were enrolled as cancer group,131 patients of precancerous lesion as CIN III group and 685 healthy individuals as control group. Three SNPs in the promoter region of adiponectin gene including rs266730( G > A),rs266729( C > G) and rs16861194( A > G) were genotyped by Taq Man method. The haplotypes were constructed and the association of these three SNPs and haplotypes with the occurrence and development of cervical cancer was analyzed. Results: In terms of both the allelic and genotypic frequency,these three SNPs( rs266730,rs16861194 and rsr266729) showed no statistically significant difference between cancer group,CIN III group and control group( P > 0. 05). The frequencies of haplotypes constructed by rs266730( G > A),rs266729( C > G) and rs16861194( A > G) also showed no statistically significant difference between cancer group,CIN III group and control group( P > 0. 05). Conclusion: These three SNPs in the adiponectin gene promoter region are not associated with the occurrence and development of cervical cancer in the Han population in Yunnan province.

关键词(KeyWords)： 宫颈肿瘤;癌前状态;多态性,单核苷酸;基因型;脂联素
cervical cancer;precancerous condition;polymorphisms,single nucleotide;genotype;adiponectin

Abstract:

Keywords:

基金项目(Foundation): 国家自然基金项目(31270030;81573206);; 协和青年基金(3332015149);; 云南省科技厅-昆明医科大学应用基础研究联合专项(2013FB169);; 云南省妇科肿瘤内设研究机构中心(2014NS032);; 中国医学科学院病原生物学研究所基本科研业务费项目(2012IPB107)

作者(Author): 张禹;严志凌;史荔;杨宏英;杨家方;俞建昆;张新文;刘舒媛;李传印;姚宇峰;
ZHANG Yu;YAN Zhiling;SHI Li;YANG Hongying;YANG Jiafang;YU Jiankun;ZHANG Xinwen;LIU Shuyuan;LI Chuanyin;YAO Yufeng;Institute of Medical Biology,Chinese Academy of Medical Sciences & Peking Union Medical College;Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Disease;The Third Affiliated Hospital of Kunming Medical University;

Email:

DOI: 10.19367/j.cnki.1000-2707.2016.07.006

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