贵州医科大学学报

2019, v.44;No.222(03) 274-278

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回转模拟失重对共培养体系中平滑肌细胞增殖及凋亡的影响
Effect of Simulated Microgravity on Proliferation and Apoptosis of Smooth Muscle Cells in Co-culture System

高原;潘益凯;李程飞;孟欣;石菲;赵疆东;杨长斌;孙喜庆;王永春;
GAO Yuan;PAN Yikai;LI Chengfei;MENG Xin;SHI Fei;ZHAO Jiangdong;YANG Changbin;SUN Xiqing;WANG Yongchun;Department of Aerospace Medical Training,School of Aerospace Medicine,Air Force Medical University;

摘要(Abstract):

目的:探讨回转模拟失重对与人脐静脉内皮细胞(HUVECs)共培养的人主动脉平滑肌细胞(HASMCs)增殖及凋亡的影响。方法:建立HUVECs与HASMCs共培养模型,以细胞回转器模拟失重效应,分为正常重力组和模拟失重组;共培养48 h时,观察HASMCs形态,采用Western blot检测2组HASMCs细胞增殖细胞核抗原(PCNA)、B淋巴细胞瘤-2(Bcl-2)及Bcl-2相关X(Bax)蛋白表达,分析模拟失重对共培养的HASMCs细胞增殖及凋亡的影响。结果:共培养48 h时,与正常重力组比较,模拟失重组HASMCs形态由长梭形变为多角形或不规则形; PCNA及Bax蛋白表达显著下降(P <0. 05)、Bcl-2蛋白表达显著增高(P <0. 05)。结论:模拟失重48 h可抑制与HUVECs共培养的HASMCs增殖和凋亡。
Objective: To investigate the effects of simulated microgravity on proliferation and apoptosis of human aortic smooth muscle cells( HASMCs) co-cultured with human umbilical vein endothelial cells( HUVECs). Methods: The co-culture model of HUVECs and HASMCs was established. The clinostat was employed to simulate microgravity. Cells were divided into normal gravity group and simulated weightless group based on their co-culture conditions. The morphology of HASMCs was observed at 48 h after co-culture,and Western blot was used to detect the expression levels of proliferating cell nuclear antigen( PCNA),B lymphocyte tumor-2( Bcl-2) and Bcl-2 associated X protein( Bax). The effects of simulated microgravity on cell proliferation and apoptosis of co-cultured HASMCs were observed. Results: Compared with the normal gravity group,the morphology of HASMCs changed from long fusiform to polygonal or irregular in simulated weightless group. The expression levels of PCNA and Bax were significantly decreased( P < 0. 05),whereas Bcl-2 protein expression was remarkably increased( P < 0. 05). Conclusion: Simulated microgravity can inhibit the proliferation and apoptosis of HASMCs co-cultured with HUVECs.

关键词(KeyWords): 失重模拟;平滑肌细胞;共培养;增殖;凋亡
weightlessness simulation;smooth muscle cells;co-culture;proliferation;apoptosis

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基金项目(Foundation): 国家自然科学基金项目(81741121,81471817)

作者(Author): 高原;潘益凯;李程飞;孟欣;石菲;赵疆东;杨长斌;孙喜庆;王永春;
GAO Yuan;PAN Yikai;LI Chengfei;MENG Xin;SHI Fei;ZHAO Jiangdong;YANG Changbin;SUN Xiqing;WANG Yongchun;Department of Aerospace Medical Training,School of Aerospace Medicine,Air Force Medical University;

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