贵州医科大学学报

2014, v.39;No.168(03) 328-332

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纤维蛋白原β链启动子区域-455单核甘酸多态性与胃癌的相关性
Study on the Relevance Between Fibrinogen β Chain Promoter Regions-455 Single Nucleotide Polymorphism and Gastric Cancer

严芝强;杨芳;王黔;王海斌;谢海涛;于扬;
YAN Zhiqiang;YANG Fang;WANG Qian;WANG Haibin;XIE Haitao;YU Yang;Gastrointestinal Surgery,the Affiliated Hospital of Guiyang Medical University;Department of Medical Laboratory,Guiyang Medical University;

摘要(Abstract):

目的:探讨纤维蛋白原(Fg)基因β链启动子区域-455G/A与胃癌发生的相关性。方法:100例胃癌患者为观察组,与观察组年龄匹配的健康体检者100例为对照组,采集两组受检者外周静脉血,用氯仿-异丙醇法提取DNA,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)和HindⅢ限制性内切酶、分别检测Fgβ-455G/A位点基因型。结果:(1)对照组Fgβ-455位点等位基因G和A的频率分别为0.745、0.255,基因型分布符合Hardy-Weinberg平衡定律;(2)胃癌组Fgβ-455位点GG、GA、AA基因型频率分别为69%、29%和2%,对照组分别为57%、35%和8%,两组比较P>0.05;Fgβ-455-G、A等位基因频率在胃癌组与对照组分别为0.835、0.745和0.165、0.255,两组比较P<0.05;(3)突变纯合子AA型与胃癌的发病有关联(OR=0.842,95%CI=0.362~9.323,P=0.034),显著降低胃癌的发病风险。结论:Fgβ-455G/A位点基因多态性可能与胃癌发病有关,A等位基因可能对胃癌发病是保护性基因。
Objective: To investigate the relevance between Fibrinogen gene beta chain promoter regions-455 gene and gastric cancer. Methods: 100 gastric cancer patients were selected as observation group,100 same age healthy controls as control group. Collecting peripheral venous blood of both groups,DNA was extracted by chloroform-isopropyl alcohol method. Then,adopting PCR-RFLP and Hind Ⅲ restriction enzymes to detect gene in BB-455-g /A polymorphism loci. Results:(1) Fgβ-455loci alleles G and A frequency were 0. 745 and 0. 255 in control group,genotype distribution was consistent with Hardy-Weinberg equilibrium principle;(2) Fgβ-455-g gene GG,GA,AA,respectively were 69%,29% and 2% in observation group,and 57% 、35% and 8% of that of control group,comparison differences P > 0. 05;(3) Fgβ-455-G,A allele were 0. 835 and 0. 745,0. 165 and 0. 255 in both groups,comparison differences P < 0. 05;(3) Homozygous mutations of AA type was relevant with the onset of gastric cancer(OR = 0. 842;95% CI was 0. 362 ~ 9. 323;P = 0. 034),which is significant in reducing the risks of gastric cancer. Conclusions: Fgβ-455 G /A polymorphism of chain promoter regions has potential relations with the onset of gastric cancer,A allele is possible to serve as protective genes for onset of gastric cancer.

关键词(KeyWords): 癌;胃;纤维蛋白原;β链启动子区域-455;多态性,单核甘酸
carcinoma;stomach;fibrinogen;beta chain promoter regions-455 gene;polymorphism,single nucleotide

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基金项目(Foundation): 贵阳市科技局,筑科合同[2012103]92号

作者(Author): 严芝强;杨芳;王黔;王海斌;谢海涛;于扬;
YAN Zhiqiang;YANG Fang;WANG Qian;WANG Haibin;XIE Haitao;YU Yang;Gastrointestinal Surgery,the Affiliated Hospital of Guiyang Medical University;Department of Medical Laboratory,Guiyang Medical University;

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DOI: 10.19367/j.cnki.1000-2707.2014.03.013

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