贵州医科大学学报

2020, v.45;No.243(12) 1382-1389+1405

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

基于生物信息学的宫颈癌枢纽基因筛选及其与预后的关系
Screening Hub Genes of Cervical Cancer Based on Bioinformatics and its Relationship with Prognosis

许修颖;龚荣府;杨娉娉;方文;
XU Xiuying;GONG Rongfu;YANG Pingping;FANG Wen;Department of Clinical Biochemistry,Guizhou Medical University;

摘要(Abstract):

目的:运用生物信息学方法筛选宫颈癌(CC)枢纽基因,并挖掘CC预后分析的潜在分子标志。方法:选取基因表达数据库(GEO)中CC表达数据集,利用R语言中的软件包筛选CC组织与正常宫颈组织中存在的差异表达基因(DEGs);并进行基因本体(GO)分析、基因组百科全书(KEGG)通路分析、蛋白质相互作用(PPI)网络构建、Cytoscape软件寻找枢纽基因、The Kaplan Meier plotter网站绘制枢纽基因生存曲线图,采用肿瘤基因组图谱(TCGA)数据库的UALCAN在线数据库验证CC患者枢纽基因的表达,采用人类蛋白质图谱(HPA)数据库分析相关蛋白的免疫组织化学结果。结果:鉴定219个上调基因和182个下调基因,KEGG通路分析结果显示差异表达基因主要富集在细胞周期、DNA复制、卵母细胞减数分裂、p53信号通路及花生四烯酸代谢等通路;筛选出的枢纽基因CDK1、KIF11、CCNB1、CDC45及CXCL8在CC中表达上调(P <0. 05),并与CC患者总体生存率(OS)相关(P <0. 05),同时在TCGA数据库中枢纽基因的表达趋势与GEO数据库中相同(P <0. 01),免疫组织化学法结果显示CXCL8未被检测到,CDK1、KIF11、CCNB1及CDC45在CC组织中表达高于正常宫颈组织。结论:筛选出CDK1、KIF11、CCNB1、CDC45及CXCL8等5个CC枢纽基因,其表达与CC的预后相关。
Objective: To screen cervical cancer( CC) hub genes with bioinformatics methods and to explore potential molecular markers for prognostic analysis of CC. Methods: The CC expression data set was downloaded from the gene expression omnibus( GEO) database,by using the package in the R language to screen differentially expressed genes( DEGs) from CC and cervical normal tissues,and systematic bioinformatics analysis of DEGs was performed,including gene ontology( GO) analysis and kyoto encyclopedia of genes and genomes( KEGG) pathway analysis,protein-protein interactions( PPI) network construction. The cytoscape software was applied to search for hub genes,and the Kaplan Meier plotter website to draw hub genes survival curve graphs. In addition,the UALCAN online database based on the cancer genome atlas( TCGA) database was used to verify the expression of pivotal genes in patients with CC,and the human protein atlas( HPA) database to analyze the immunohistochemical results of related proteins. Result: 219 up-regulated genes and 182 downregulated genes were identified. KEGG pathway analysis results showed that DEGs were mainly enriched in the cell cycle,DNA replication,oocyte meiosis,p53 signaling pathway and arachidonic acid metabolism and other pathways. The selected hub genes CDK1,KIF11,CCNB1,CDC45,and CXCL8 were up-regulated in CC( P < 0. 05),and related to the overall survival rate( OS) of patients with CC( P < 0. 05). The expressional trends of the pivot genes in the TCGA database were the same as in the GEO database. Immunohistochemical results showed that CXCL8 was not detected,and the expressions of CDK1,KIF11,CCNB1,and CDC45 in CC tissues were higher than those in normal cervical tissues. Conclusion: Five hub genes of CC,namely CDK1,KIF11,CCNB1,CDC45,and CXCL8 are screened,and their expressions are related to the prognosis of CC.

关键词(KeyWords): 宫颈肿瘤;计算生物学;预后;差异表达基因;枢纽基因;KEGG通路
cervical cancer(CC);computational biology;prognosis;differentially expressed genes(DEGs);hub genes;KEGG pathway

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81560481)

作者(Author): 许修颖;龚荣府;杨娉娉;方文;
XU Xiuying;GONG Rongfu;YANG Pingping;FANG Wen;Department of Clinical Biochemistry,Guizhou Medical University;

Email:

DOI: 10.19367/j.cnki.2096-8388.2020.12.004

参考文献(References):

文章评论(Comment):

序号(No.) 时间(Time) 反馈人(User) 邮箱(Email) 标题(Title) 内容(Content)
反馈人(User) 邮箱地址(Email)
反馈标题(Title)
反馈内容(Content)
扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享