贵州医科大学学报

2018, v.43;No.215(08) 904-908+923

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饮用某54% vol白酒对大鼠肾上腺髓质细胞ADM及ACTH表达的影响
Effect of 54% vol Liquor on Expression of ADM and ACTH in Adrenal Medulla Cell of Rats

陈兴强;李容;尹丹;李杰;夏白娟;梁文妹;
CHEN Xingqiang;LI Rongrong;YIN Dan;LI Jie;XIA Baijuan;LIANG Wenmei;Department of Histology and Embryology,Guizhou Medical University;

摘要(Abstract):

目的:探讨摄入不同剂量某54%vol白酒对大鼠肾上腺髓质细胞内肾上腺髓质素(ADM)及促肾上腺皮质激素(ACTH)表达的影响。方法:正常雄性SD大鼠120只随机法分为正常对照组(NG,n=30)和实验组(灌胃白酒,n=90),实验组又分为低剂量组[LG,0.8 m L/(kg·d)]、中剂量组[MG,1.6 m L/(kg·d)]和高剂量组[HG,2.4 m L/(kg·d)],实验组大鼠于每日11∶00 AM及5∶00 PM各灌胃一次,NG组不予处理;各组大鼠分别于实验第4、8、12、16及20周末取肾上腺组织,采用免疫组织化学SABC法、图像分析法、Western blot检测大鼠肾上腺髓质中ADM及ACTH表达。结果:与NG相比较,第4、8、12、16及20周末各实验组,大鼠肾上腺髓质细胞ADM、ACTH免疫组织化学染色强度及平均光密度差异均无统计学意义(P>0.05);Western blot显示,与NG组比较,第4、8、12、16及20周各实验组大鼠肾上腺组织ADM、ACTH蛋白表达水平差异均无统计学意义(P>0.05)。结论:在本实验设定的剂量和时长下,摄入该54%vol白酒对大鼠肾上腺髓质ADM及ACTH的表达无明显影响。
Objective: To investigate the effects of different doses of certain 54% vol Liquor on the expression of adrenomedullin( ADM) and adrenocorticotropic hormone( ACTH) in rat adrenal medulla cells. Methods: 120 normal male SD rats were randomly divided into normal control group( NG,n= 30) and experimental group( EG,n = 90). The experimental group was divided into low dose group[LG,0. 8 mL/( kg·d) ],middle dose group [MG,1. 6 mL/( kg·d) ]and high-dose group [HG,2. 4 mL/( kg·d) ]. Rats in the experimental group were given intragastric administration at 11∶ 00 a.m. and 5∶ 00 p. m. daily,but no treatment was given in the normal group. The adrenal tissues of each group were collected at the end of the 4 th,8 th,12 th,16 th and 20 th week. The expression of ADM and ACTH in the adrenal medulla was detected by immunohistochemical SABC method,image analysis method and Western blot. Results: The immunoreactive products of ADM and ACTH in normal and experimental groups were brown or claybank,and distributed in the adrenal medulla cytoplasm/karyon. At the end of the 4 th,8 th,12 th,16 th and 20 th week.,there was no significant difference in immunohistochemical staining intensity and average optical density of adrenal medulla cells between the experimental group and the normal control group( P > 0. 05). Western blot showed that there was no significant difference in the expression of ACTH protein in adrenal tissue of rats in the 4 th,8 th,12 th,16 th and 20 th week compared with the normal control group( P > 0. 05). Conclusion: Under the dosage and duration of the experiment,the expression of ADM and ACTH in the adrenal medulla of rats was not significantly affected by the intake of the 54% vol liquor.

关键词(KeyWords): 食用白酒;肾上腺髓质素;促肾上腺皮质激素;肾上腺髓质;免疫组织化学;免疫印记
liquor;adrenomedullin;adrenocorticotropic hormone;adrenal medulla;Immunohistochemistry;Western blot

Abstract:

Keywords:

基金项目(Foundation): 贵州省教育厅“125”重大科技专项基金资助[黔教合重大专项字(2012)007]

作者(Author): 陈兴强;李容;尹丹;李杰;夏白娟;梁文妹;
CHEN Xingqiang;LI Rongrong;YIN Dan;LI Jie;XIA Baijuan;LIANG Wenmei;Department of Histology and Embryology,Guizhou Medical University;

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DOI: 10.19367/j.cnki.1000-2707.2018.08.008

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