贵州医科大学学报

2014, v.39;No.171(06) 868-872+876

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miR-125a和miR-206在乳腺癌中的表达及临床意义
Expressions of miR-125a and miR-206 in Patients with Breast Cancer and Its Clinical Significance

闵卫利;李杰;韩佳;赵阳;代志军;王西京;马清涌;
MIN Weili;LI Jie;HAN Jia;ZHAO Yang;DAI Zhijun;WANG Xijing;MA Qingyong;Department of Oncology,the Second Affiliated Hospital of Xi'an Jiaotong University;Medical Board,Xi'an Jiaotong University;Department of Hepatobiliary Surgery,the First Affiliated Hospital of Xi'an Jiaotong University;

摘要(Abstract):

目的:探讨miR-125a和miR-206在乳腺癌中的表达及临床意义。方法:应用Real-time PCR检测35例乳腺癌组织及其癌旁乳腺组织中miR-125a和miR-206的表达,用免疫组化检查乳腺癌中雌激素受体(ER)和孕激素受体(PR)的表达,荧光原位杂交技术检测人类表皮生长因子受体2(HER-2)和DNA拓扑异构酶2A(TOP2A)表达;分析miR-125a和miR-206与乳腺癌患者年龄、月经、淋巴结状态、HER-2、TOP 2A、ER、PR的表达等临床病理特征的相关性。结果:miR-125a和miR-206在乳腺癌组织中的表达水平显著低于癌旁组织,miR-125a高表达与淋巴结阴性、HER-2阴性、TOP 2A阴性具有相关性(P<0.05),与乳腺癌患者肿瘤大小、年龄、月经状态、ER、PR无相关性。miR-206的高表达与较小的肿瘤体积、ER阴性、PR阴性、HER-2阴性具有相关性(P<0.05),与乳腺癌患者年龄、月经、淋巴结状态及TOP 2A的表达程度无关。结论:miR-125a和miR-206在乳腺癌发生发展过程中可能发挥重要作用,对指导HER-2阳性和三阴性乳腺癌的治疗有重要临床意义。
Objective: To investigate the expressions of miR-125 a and miR-206 in breast cancer and its clinical significance. Methods: Real-time RT-PCR was adopted to detect miR-125 a and miR-206 expressions in breast cancer tissues and adjacent normal breast tissues of 35 cases; The expressions of estrogen receptor( ER) and progesterone receptor( PR) were examined by immunohistochemistry,Fluorescence in situ hybridization( FISH) was used to detect of HER-2 and TOP 2A expression. The relationship of miR-125 a and miR-206 expression with clinical and pathological features such as age,menstruation,lymph node status,the expression of HER-2,TOP 2A,ER,PR of breast cancer patients was analyzed. Results: miR-125 a and miR-206 expressions in breast cancer tissuses were lower than those in the adjacent normal tissues. miR-125 a high expression was correlated with negative status of lymph node,HER-2 and TOP 2A( P < 0. 05) and was not correlated with tumor size,age,menstruation,ER and PR levels. miR-206 high expression was correlated with smaller tumor sizes,negative status of ER,PR-negative status,HER-2,and was not correlated with age,menstruation,lymph node metastasis and TOP 2A expression. Conclusions: miR-125 a and miR-206 may play an important role in carcinogenesis and aggressiveness of breast cancer and can guide the treatment of HER-2-positive and triple negative breast cancer.

关键词(KeyWords): 癌;乳腺肿瘤;miR-125a;miR-206;受体,雌激素;受体,孕激素
cancer;breast neoplasms;miR-125a;miR-206;receptors,estrogen;receptors,progesterone

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基金项目(Foundation): 西安市社会发展引导计划[SF1203(4)]

作者(Author): 闵卫利;李杰;韩佳;赵阳;代志军;王西京;马清涌;
MIN Weili;LI Jie;HAN Jia;ZHAO Yang;DAI Zhijun;WANG Xijing;MA Qingyong;Department of Oncology,the Second Affiliated Hospital of Xi'an Jiaotong University;Medical Board,Xi'an Jiaotong University;Department of Hepatobiliary Surgery,the First Affiliated Hospital of Xi'an Jiaotong University;

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