陈华妹;李铮;王旭东;王红梅;秦奕男;朱筑霞;
CHEN Huamei;LI Zheng;WANG Xudong;WANG Hongmei;QIN Yinan;ZHU Zhuxia;Department of Physiology,Guiyang Medical University;

• 1:贵州医科大学生理学教研室

摘要(Abstract)：

目的:探讨钙蛋白酶-周期蛋白E(Calpain-Cyclin E)信号通路在表皮生长因子(EGF)诱导的乳腺癌细胞迁移中的介导作用。方法:取对数生长期MCF-7乳腺癌细胞系为模型细胞,采用EGF、钙离子螯合剂(BAPTA)、钙蛋白酶抑制剂(Calpeptin)及钙蛋白酶抑制剂I(ALLN)预处理模型细胞,通过伤口愈合实验观察细胞迁移能力,通过蛋白印迹法观察蛋白质剪切及分子量变化,观察Calpain-Cyclin E信号通路对EGF诱导的MCF-7乳腺癌细胞迁移能力的影响。结果:BAPTA及ALLN能显著阻断EGF诱导的MCF-7乳腺癌细胞迁移,BAPTA组与Con组相比P<0.01,ALLN组与EGF组相比P<0.01,差异具有统计学意义;Calpeptin能阻断EGF诱导的细胞CalpainⅠ、Cyclin E蛋白剪切。结论:Calpain-Cyclin E促进EGF诱导的MCF-7乳腺癌细胞的迁移能力加强,可能在乳腺癌的浸润生长中发挥作用。
Objective: To investigate the effect of the Calpain /Cyclin E pathways in epidermal growth factor( EGF)-induced migration in MCF-7 breast cancer cells. Methods: Using MCF-7 breast cancer cells as a model,EGF,Chelators( BAPTA) and CANP inhibitors( Calpeptin / ALLN) were applied for pretreatment of model cells. Cell migration was determined by wound healing assay. Western blot was used to analyze protein expression changes. The effect of Calpain / Cyclin E signaling on EGF-induced migration in breast cancer cells was investigated. Results: BAPTA and ALLN could prevent EGF-induced migration of MCF-7 breast cancer,compared between BAPTA group and control group( P <0. 01),compared between EGF group and ALLN group( P < 0. 01); Calpeptin was able to prevent EGF-induced Cyclin E processing and migration in breast cancer cells. Conclusion: Ca2 +-Calpain-Cyclin E signaling enhances EGF-induced migration in MCF-7 breast cancer cells.

关键词(KeyWords)： 表皮生长因子;钙蛋白酶;周期蛋白E;乳腺肿瘤;迁移
epidermal growth factor;calpain;cyclin E;breast cancer;migration

Abstract:

Keywords:

基金项目(Foundation): 黔科合SY字[2012]3100号

作者(Author): 陈华妹;李铮;王旭东;王红梅;秦奕男;朱筑霞;
CHEN Huamei;LI Zheng;WANG Xudong;WANG Hongmei;QIN Yinan;ZHU Zhuxia;Department of Physiology,Guiyang Medical University;

Email:

DOI: 10.19367/j.cnki.1000-2707.2015.12.015

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