贵州医科大学学报

2018, v.43;No.211(04) 400-405

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芳姜黄酮及其衍生物对α-葡萄糖苷酶和α-淀粉酶活性的抑制作用
Inhibitory Effect of Ar-turmerone and Its Derivatives on Activity of α-Glucosidase and α-Amylase

肖露;陈兰;荣冬芸;王叶;曹煜;吴春维;
XIAO Lu;CHEN Lan;RONG Dongyun;WANG Ye;CAO Yu;WU Chunwei;Guizhou Medical University;Department of Physical Examination,the Affiliated Hospital of Guizhou Medical University;

摘要(Abstract):

目的:研究芳姜黄酮及其衍生物对α-葡萄糖苷酶和α-淀粉酶活性的影响。方法:分别以4-硝基苯-β-D-吡喃葡萄糖苷(PNPG)和可溶性淀粉为底物,测定芳姜黄酮及其衍生物对α-葡萄糖苷酶和α-淀粉酶活性的抑制率,分析对α-葡萄糖苷酶的抑制类型。结果:芳姜黄酮及其衍生物能够以剂量依赖性、时间依赖性的方式抑制α-葡萄糖苷酶和α-淀粉酶的活性,且对α-葡萄糖苷酶活性的抑制类型为竞争性抑制;计算结果显示,衍生物PY-2-17、PY-2-33和阿卡波糖的IC50值分别为0.397、0.314和0.57 g/L。结论:芳姜黄酮及其衍生物能够抑制ɑ-葡萄糖苷酶和α-淀粉酶活性,且其衍生物PY-2-17和PY-2-33对α-葡萄糖苷酶活性的抑制作用优于阿卡波糖。
Objective: To determine the effect of ar-turmerone and its derivatives on the activity of α-glucosidase and α-amylase. Methods: Using p-nitrophenyl-β-D-galactopyranoside( PNPG) and soluble starch solution as substrate respectively to determine the inhibitory rate of ar-turmerone and its derivatives on α-glucosidase and α-amylase,and its inhibitory type on α-glucosidase. Results: Ar-turmerone and its derivatives exhibited inhibition against α-glucosidase and α-amylase with time and dose-dependent feature. Moreover,the inhibitory type was competitive against the activity ofα-glucosidase.Calculation indicated that the derivatives PY-2-17,PY-2-33 and Acasbose IC50 values were0. 397 g/L,0. 314 g/L and 0. 57 g/L respectively. Conclusions: Ar-turmerone and its derivatives demonstrated the inhibitory effect against activity of ɑ-glucosidase and α-amylase,in which the derivatives PY-2-17 and PY-2-33 are superior to Acasbose with α-glucosidase inhibition.

关键词(KeyWords): 芳姜黄酮;芳姜黄酮衍生物;α-葡萄糖苷酶;α-淀粉酶;抑制
ar-turmerone;ar-turmerone derivatives;α-glucosidase;α-amylase;inhibition

Abstract:

Keywords:

基金项目(Foundation): 贵州省产业技术创新战略联盟项目[黔科合平台人才(2016)5501号];; 贵阳市科技局科技类项目[筑科合同(20161001)号]

作者(Author): 肖露;陈兰;荣冬芸;王叶;曹煜;吴春维;
XIAO Lu;CHEN Lan;RONG Dongyun;WANG Ye;CAO Yu;WU Chunwei;Guizhou Medical University;Department of Physical Examination,the Affiliated Hospital of Guizhou Medical University;

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DOI: 10.19367/j.cnki.1000-2707.2018.04.006

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