牛雪可;邱文;兰永哲;李文均;廖万清;沈志平;余能彬;邹成刚;康颖倩;
NIU Xueke;QIU Wen;LAN Yongzhe;LI Wenjun;LIAO Wanqing;SHEN Zhiping;YU Nengbin;ZOU Chenggang;KANG Yingqian;Department of Microbiology, School of Basic Medical Sciences, Guizhou Medical University;Guizhou Talent Base Program of Guizhou Provincial Government & Key Laboratory of Medical Microbiology and Parasitology of of Colleges and Universities in Guizhou Province, School of Basic medical Sciences, Guizhou Medical University;School of Life Sciences, Sun Yat-sen University;Institute of Medical Myco

• 1:贵州医科大学基础医学院微生物学教研室
• 2:贵州省微生物与人类健康关系研究人才基地暨贵州省普通高校病原生物学特色重点实验室
• 3:贵州医科大学
• 4:中山大学生命科学学院

摘要(Abstract)：

目的:研究贵州省某未开发热泉中可培养细菌的多样性及在不同温度胁迫下细菌的变化情况。方法:采用R2A培养基,分别置于70、65、60、55、50、45、37以及25℃下对热泉细菌进行培养,涂布法对所培养细菌进行分离鉴定,采用最大拟自然法法构建16s rRNA系统发育树,比较不同温度胁迫下,可培养细菌多样性的差异。结果:共分离得到243株可培养细菌,归属于25个种、20个属、5个纲以及3个门;在属间水平上,α-变形菌纲细菌的多样性最为丰富;在菌株数量上,α-变形菌纲属于优势类群;不同温度培养下,所得到的优势菌不同,在60、55、50、45、37以及25℃6种培养温度下分离出的菌株在群落分布上呈现明显的差异,37℃细菌群落多样性最丰富。结论:该热泉中微生物多样性丰富,未发现的新类群;温度对群落的组成有显著影响,温度可能与生物多样性变化相关。
Objective: To investigate the diversity of culturable bacteria in an undeveloped hot spring in Guizhou Province and evaluate the effect of temperatures on the diversity of these culturable bacteria. Methods: R2A medium was used to culture hot spring bacteria at 70, 65, 60, 55, 50, 45, 37 and 25 ℃. The cultured bacteria were isolated by the coating method. The isolated strains were identified based on 16S rRNA gene sequence. The 16s rRNA phylogenetic tree was constructed using Maximum-likelihood method. The differences in bacterial diversity under different temperature stresses were compared. Results: A total of 243 culturable bacteria were isolated, belonged to 25 species, 20 genera, 5 classes and 3 gates. At the intergeneric level, α-Proteobacteria were the most diverse and also dominant. the dominant bacteria obtained from culture are different under different temperatures. The strains isolated from 60, 55, 50, 45, 37 and 25 ℃ cultures were remarkably different in the community distribution. The diversity of the bacterial community cultured under 37 ℃ is the most abundant. Conclusion: The hot spring is rich in microbial diversity, but not any new group is found; the temperature has a significant effect on the composition of the bacterial community. The temperature may be related to biodiversity of these culturable bacteria.

关键词(KeyWords)： 热泉;细菌;培养;生物多样性;温度胁迫;贵州
hot spring;bacteria;culture;biodiversity;temperature stress;Guizhou

Abstract:

Keywords:

基金项目(Foundation): 贵州省科技厅优秀青年科技人才培养计划项目[黔科合(20175639)];贵州省科技厅科技支撑计划项目[黔科合支撑(2019)2873];; 贵州省卫计委医疗卫生援黔专家团廖万清院士工作室项目(gzwjkj2016-1-050);; 贵阳市科技局项目[筑科合同(2017)5-19]

作者(Author): 牛雪可;邱文;兰永哲;李文均;廖万清;沈志平;余能彬;邹成刚;康颖倩;
NIU Xueke;QIU Wen;LAN Yongzhe;LI Wenjun;LIAO Wanqing;SHEN Zhiping;YU Nengbin;ZOU Chenggang;KANG Yingqian;Department of Microbiology, School of Basic Medical Sciences, Guizhou Medical University;Guizhou Talent Base Program of Guizhou Provincial Government & Key Laboratory of Medical Microbiology and Parasitology of of Colleges and Universities in Guizhou Province, School of Basic medical Sciences, Guizhou Medical University;School of Life Sciences, Sun Yat-sen University;Institute of Medical Myco

Email:

DOI: 10.19367/j.cnki.1000-2707.2019.05.004

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