贵州医科大学学报

2019, v.44;No.228(09) 1029-1033

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干扰素对人Burkitt's淋巴瘤细胞Raji增殖的影响及机制
Effect of Interferon on Proliferation of Raji Cells and Its Mechanism

谭大为;郑丹;陈秀萍;
TAN Dawei;ZHENG Dan;CHEN Xiuping;Department of Hematology, the Affiliated Baiyun Hospital of Guizhou Medical University;Guiyang Nursing Vocational College;Qixingguan Hospital,Bijie;

摘要(Abstract):

目的:探讨干扰素对人Burkitt's淋巴瘤细胞Raji增殖的影响及机制。方法:采用100、500、1 000、2 000及5 000 U/mL干扰素分别处理Raji细胞12、24及48 h,采用CCK-8法检测Raji细胞的增殖活性,实时荧光定量PCR扩增(QPCR)检测各浓度干扰素处理Raji细胞48 h时BZLFl、BRLFlmRNA表达水平。结果:各浓度干扰素对Raji细胞的增殖均有一定的抑制作用,且随着干扰素浓度的增加,其抑制作用逐渐增强,各浓度组间比较差异有统计学意义(P<0.05);各浓度干扰素均能上调Raji细胞BZLFl及BRLFlmRNA表达,且随着干扰素浓度增加,基因的表达量增多,各浓度组间比较差异有统计学意义(P<0.05)。结论:干扰素能抑制Raji细胞增殖、且呈浓度依赖性,其机制可能是通过上调BZLFl及BRLFl基因的表达来抑制Raji细胞的增殖。
Objective: To investigate the effect of interferon on the proliferation and expression of BZLFl mRNA, BRLFl mRNA in Raji cells and its mechanism.Methods: Raji cells were cultured and treated with 100, 500, 1 000, 2 000, 5 000 U/mL interferon for 12, 24, 48 h respectively.Then the cell proliferation was tested with CCK 8 assay. The expression of BZLFl and BRLFl mRNA in Raji cells treated with interferon at various concentrations for 48 h was detected by QPCR.Results: At the different concentrations of 100~5 000 U/mL, interferon effectively inhibited Raji cells proliferation and its inhibitory effect was gradually enhanced with the increase of interferon concentration, the difference between groups with different concentrations was statistically significant(P<0.05). All the selected interferon concentrations increased the expression of BZLFl mRNA, BRLFl mRNA in a dose-dependent manner. The expression of genes increased with the increasing of interferon concentration, and the difference between the groups with different concentrations was statistically significant(P<0.05). Conclusion: Interferon can inhibit proliferation by upregulating the expression of BZLFl mRNA and BRLFl mRNA in Raji cells.

关键词(KeyWords): 淋巴瘤;EB病毒;Raji细胞;干扰素;增殖;抑制;BZLFl基因;BRLFl基因
lymphoma;EB virus;Raji cells;interferon;proliferation;inhibitation;BZLFl gene;BRLFl gene

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基金项目(Foundation): 贵州省科学技术厅基金[黔科合LH字(2015)7398]

作者(Author): 谭大为;郑丹;陈秀萍;
TAN Dawei;ZHENG Dan;CHEN Xiuping;Department of Hematology, the Affiliated Baiyun Hospital of Guizhou Medical University;Guiyang Nursing Vocational College;Qixingguan Hospital,Bijie;

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DOI: 10.19367/j.cnki.1000-2707.2019.09.008

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