贵州医科大学学报

2017, v.42;No.200(05) 592-594+599

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血细胞分析仪在脑脊液白细胞分类中的应用
Clinical Application of Blood Cell Analyzer in the Classification of Cerebrospinal Fluid Leukocyte

丛硕;毕瑩;
CONG Suo;BI Ying;Department of Blood Transfusion,Guizhou Provincial Tumor Hospital;School of Clinical Laboratory,Guizhou Medical University;

摘要(Abstract):

目的:探讨Sysmex XE-5000血细胞分析仪(血细胞分析仪)用于检测脑脊液中白细胞(WBC)分类的效果。方法:取3份已知WBC计数为高、中、低浓度的脑脊液标本,在室温下用血细胞分析仪连续测定10次,项目包括单个核细胞(MN)和多个核细胞(PMN)百分比,用标准差(SD)与变异系数(CV)评价血细胞分析仪的批内精密度;再另取1份WBC计数>30×106/L的脑脊液标本用血细胞分析仪连续检测20次,计算SD和CV评价血细胞分析仪的批间精密度,反映仪器的重复性;采用血细胞分析仪和手工瑞氏染色法后显微镜镜检法分别对80份脑脊液标本进行WBC分类计数,采用Pearson法分析其相关性。结果:血细胞分析仪对脑脊液WBC分类的批内和批间CV<30%,说明本次使用的血细胞分析仪对脑脊液WBC分类的批内和批间精密度在允许的范围,并具有良好的重复性;分别用血细胞分析仪和手工瑞氏染色镜检法检测80例脑脊液样本,两种方法测定的MN和PMN百分比比较,差异无统计学意义(P>0.05),相关分析显示两组检测结果存在正相关(r>0.9,P<0.05)。结论:Sysmex XE-5000血细胞分析仪可用于脑脊液WBC分类计数。
Objective: To investigate the effect of blood cell analyzer,Sysmex XE-5000,in the classification of cerebrospinal fluid leukocyte(WBC). Methods: Taking three white blood cell count which was known for high,medium and low concentration of cerebrospinal fluid specimens,using blood cell analyzer to detect 10 times in a row at room temperature with standard deviation(SD) and coefficient of variation(CV) to evaluate precision of the analyzer; then another sample of white blood cell count which was greater than 30 × 106/L were detected by blood cell analyzer for 20 times; counting precision between SD and CV to evaluate the precision of analyzer and reflected the repeatability of instrument. 80 cerebrospinal fluid specimens were counted on leukocyte categories and compared by analyzer and first manual staining microscopy and then visual detection. Results: It was indicated that within-batch and inter-batch CV < 30% from classification of analyzer on cerebrospinal fluid WBC,which proved that the blood cell analyzer exhibited precision of within-batch and inter-batch in acceptable range with fine repeatability; 80 cerebrospinal fluid specimens were detected by blood cell analyzer and manual staining microscopy and then visual detection method respectively,MN and PMN percentage comparison of both methods showed no statistical significance(P > 0. 05),relevant analysis indicated that both test results were positively correlated(r > 0. 9,P < 0. 05). Conclusion: the blood cell an-alyzer can be used as a fast and exact classification of cerebrospinal fluid leukocyte.

关键词(KeyWords): 脑脊液;白细胞;分类计数,多个核细胞;血细胞分析仪;手工法
cerebrospinal fluid;white blood cell;classification count,multiple nuclear cells;hematology analyzer;manual method

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作者(Author): 丛硕;毕瑩;
CONG Suo;BI Ying;Department of Blood Transfusion,Guizhou Provincial Tumor Hospital;School of Clinical Laboratory,Guizhou Medical University;

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