贵州医科大学学报

2014, v.39;No.171(06) 800-804

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生理层流剪切力对未成熟树突状细胞microRNA表达谱的影响
The Effect of Physiological Laminar Flow-Derived Shear Stress on the microRNA Profiles of Immature Dendritic Cells

董蓉;薛慧;杨晖;龙金华;胡祖权;张春林;姚伟娟;曾柱;
DONG Rong;XUE Hui;YANG Hui;LONG Jinhua;HU Zuquan;ZHANG Chunlin;YAO Weijuan;ZENG Zhu;Teaching Division of Bio-technology,School of Biology and Engineering,Guiyang Medical University;Department of Head and Neck Oncology,Guiyang Tumor Hospital;Teaching Division of Biology,School of Basic Medicine,Guiyang Medical University;Hemorheology Center,School of Basic Medicine,Peking University;

摘要(Abstract):

目的:探讨生理层流剪切力对未成熟树突状细胞(im DCs)microRNA表达谱的影响。方法:用锥板剪切系统加载10 dyn/cm2的剪切力作用于im DCs 1 h,对照组不加载剪切力,基因芯片技术检测microRNA的表达变化并对其结果进行生物信息学分析。结果:与对照组相比,im DCs在层流剪切力作用后有25个microRNAs表达发生变化,变化倍数>1.5,差异有统计学意义(P<0.05);生物信息学分析显示这些变化的microRNA的靶基因主要参与免疫功能调控和细胞对流体剪切力应答相关的信号通路。结论:层流剪切力能够影响im DCs microRNA的表达谱,说明物理因素(剪切力)可能参与了机体的免疫功能调控。
Objective: To investigate the effect of laminar flow-derived shear stress on the microRNA expression profiles of immature dendritic cells( im DCs). Methods: The cultured im DCs were loaded with 10 dyn / cm2 laminar flow shear stress for 1 h in cone-and-plate device and control group was not loaded. microRNAs were isolated and investigated by genome-wide microarray,and then the results were analyzed with bioinformatics. Results: The data showed that the expression levels of 25 microRNAs in im DCs were changed by shear stress( fold change > 1. 5,P < 0. 05). Further analysis with bioinformatics indicated that the target genes were mainly involved in regulation of immune function and the signaling pathway of cellular response to shear stress. Conclusion: The microRNA expression profiles of im DCs can be affected by shear stress,which suggests that physical factors( shear stress) may be involved in the regulation of immune response.

关键词(KeyWords): 树突状细胞;流体剪切力;microRNA;基因芯片;生物信息学;基因表达谱
dendritic cells;laminar flow-derived shear stress;microRNA;genome-wide microarray;bioinformatics;gene expression profile

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基金项目(Foundation): 国家自然科学基金(11162003,31260227,31170886);; 教育部科学技术研究重点项目(210196);; 贵州省优秀青年科技人才支持计划(2011-24);; 贵州省社会发展科技攻关项目[黔科合SY字(2011)3065];; 贵州省省长专项基金[黔省专合字(2009)-79];; 贵州省科学技术基金[黔科合J字2008-2274和(2013)2058];; 贵阳市科学技术项目(2010-筑科农合同字第1-社-12);; 贵州省留学回国人员科技活动基金(2013-8)

作者(Author): 董蓉;薛慧;杨晖;龙金华;胡祖权;张春林;姚伟娟;曾柱;
DONG Rong;XUE Hui;YANG Hui;LONG Jinhua;HU Zuquan;ZHANG Chunlin;YAO Weijuan;ZENG Zhu;Teaching Division of Bio-technology,School of Biology and Engineering,Guiyang Medical University;Department of Head and Neck Oncology,Guiyang Tumor Hospital;Teaching Division of Biology,School of Basic Medicine,Guiyang Medical University;Hemorheology Center,School of Basic Medicine,Peking University;

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