贵州医科大学学报

2016, v.41;No.191(08) 882-886

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阿托伐他汀对脂多糖诱导人主动脉内皮细胞细胞间黏附分子-1表达的影响
Effect of Atorvastatin on the Expression of Intercellular Adhesion Molecule-1 in Cultured Human Aortic Endothelial Cells Induced by Lipopolysaccharide

嵇云鹏;张彦燕;杨红;石廷雨;徐旖旎;陶玲;沈祥春;
JI Yunpeng;ZHANG Yanyan;YANG Hong;SHI Tingyu;XU Yini;TAO Ling;SHEN Xiangchun;The Key Lab.of Optimal Utilization of Natrual Medicine Resources,Guizhou Medical University;Department of Pharmacy,Guizhou Provincial People's Hospital;Department of Pharmacology of Traditional Chinese Medicine,Guizhou Medical University;

摘要(Abstract):

目的:观察阿托伐他汀(Atv.)对脂多糖(LPS)诱导人主动脉内皮细胞(HAECs)细胞间黏附分子-1(ICAM-1)表达的影响,并探讨其分子机制。方法:用1 mg/L LPS复制HAECs损伤模型,通过MTT和乳酸脱氢酶(LDH)外漏率实验观察5μmol/L Atv.对LPS诱导HAECs损伤的保护作用,采用RT-PCR法检测Atv.对ICAM-1 mRNA表达的影响,Western blot检测Atv.对ICAM-1、IκBα及NF-κB蛋白表达水平的影响。结果:与LPS组比较,Atv.能有效抑制LPS诱导的HAECs损伤,细胞活力增加(P<0.01),LDH外漏率减少(P<0.01),Atv.可抑制LPS诱导的ICAM-1 mRNA(P<0.05)和蛋白表达上调(P<0.01),还可抑制LPS诱导的IκBα蛋白表达水平的降低和phospho-NF-κB p65蛋白表达水平的升高(P<0.01)。结论:阿托伐他汀对LPS诱导HAECs损伤具有保护作用,并有效抑制ICAM-1的表达,该作用与抑制IκBα降解及NF-κB p65磷酸化有关。
Objective: To observe the effect and mechanism of atorvastatin on the expression of ICAM-1 in lipopolysaccharide( LPS)-induced injury of cultured human aortic endothelial cells( HAECs). Methods: Cultured HAECs were treated with LPS alone( 1 mg /L) or in the presence of atorvastatin( Atv.,5 μmol / L). MTT and LDH leakage ratio were used to analyze the protective effect of atorvastatin against LPS. The RT-PCR was used to determine the mRNA expression of ICAM-1. The western blot was adopted to detect the protein expression of ICAM-1,IκBα,NF-κB in HAECs injury induced by LPS. Results: Compared with LPS group,atorvastatin could effectively inhibit LPS-induced injury by increasing cell activity( P < 0. 01),decreasing LDH releasing ratio( P < 0. 01),inhibiting the up-regulating mRNA( P < 0. 05) and protein( P < 0. 01) expression of ICAM-1,inhibiting the decreasing of phospho-NF-κB p65 protein expression( P < 0. 01) and the increasing of IκBα pro-tein expression( P < 0. 01). Conclusion: Atorvastatin has a protective effect on LPS induced HAECs injury,and can effectively inhibit the expression of ICAM-1,which is related to the inhibition of IκBαdegradation and NF-κB p65 phosphorylation.

关键词(KeyWords): 阿托伐他汀;人主动脉内皮细胞;细胞间黏附分子-1;核因子-κB
atorvastatin;human aortic endothelial cells;intercellular adhesion molecule-1;nuclear factor-κB

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(81360650;81560811);; 贵州省留学人员科技活动项目[黔人资合(2013)02号];; 贵州省高层次创新型人才[黔科合人才(2015)4029号];; 贵阳市现代药业计划[筑科合同(2011204)17号];; 贵州省中药现代化专项[黔科合中药字(2012)5051号];; 贵州省高等学校创新团队[黔教合人才团队(2014)31号];; 贵州省创新团队[黔科合人才团队(2015)4025号]

作者(Author): 嵇云鹏;张彦燕;杨红;石廷雨;徐旖旎;陶玲;沈祥春;
JI Yunpeng;ZHANG Yanyan;YANG Hong;SHI Tingyu;XU Yini;TAO Ling;SHEN Xiangchun;The Key Lab.of Optimal Utilization of Natrual Medicine Resources,Guizhou Medical University;Department of Pharmacy,Guizhou Provincial People's Hospital;Department of Pharmacology of Traditional Chinese Medicine,Guizhou Medical University;

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DOI: 10.19367/j.cnki.1000-2707.2016.08.004

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