贵州医科大学学报

2016, v.41;No.192(09) 1033-1036

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内质网应激前后肝癌细胞中C/EBP同源蛋白及X-盒-结合蛋白-1的表达
The Expression of XBP-1 and CHOP Alteration in the Normal Liver Cells and Liver Cancer Cells before and after RES

李利波;潘娅;陈腾祥;
LI Libo;PAN Ya;CHEN Tengxiang;Guizhou Medical University;

摘要(Abstract):

目的:观察内质网应激(ERS)标志蛋白X-盒-结合蛋白-1(XBP-1)和C/EBP同源蛋白(CHOP)在正常肝细胞及不同分化程度肝癌细胞中的表达。方法:将培养获得的正常肝细胞LO2、高分化肝癌细胞株Hep G2及低分化肝癌细胞株SMMC-7721细胞分为无水乙醇未处理组(ERS前组)和无水乙醇处理组(ERS组),ERS组的3种细胞分别给予无水乙醇处理获得ERS细胞模型;采用Western Blot方法检测3种细胞ERS前后XBP-1、CHOP表达。结果:在ERS前组中,XBP-1表达从高到低依次为LO2、Hep G2、SMMC-7721;ERS组中,XBP-1在3种细胞中的表达都上调,与ERS前组比较差异有统计学意义(P<0.05),增加量从高到低依次为SMMC-7721、LO2及Hep G2,3种细胞间两两比较差异无统计学意义(P>0.05);ERS前后,Hep G2细胞中均无CHOP表达;ERS前组中,CHOP表达高低依次为SMMC-7721、LO2;ERS组中,CHOP在LO2细胞及SMMC-7721细胞表达均上调,与ERS前组比较差异有统计学意义(P<0.05),而在SMMC-7721细胞中表达上调更明显(与LO2细胞比较,P<0.05)。结论:发生ERS后,LO2、Hep G2及SMMC-7721细胞中XBP-1的表达水平都升高,分化程度低的SMMC-7721细胞中的XBP-1及CHOP的表达升高更明显。
Objective: To observe the alteration of ERS marker protein X-box-binding protein 1( XBP-1) and C /EBP homologous protein( CHOP) in normal liver cells LO2,expression of high differentiation of primary liver cancer cell line Hep G2 and low expression in the differentiation of primary liver cancer cell line SMMC-7721. Methods: Cultivation of LO2 cells and Hep G2,SMMC-7721 cells was divided into anhydrous ethanol untreated group before( ERS) and anhydrous ethanol treatment group( ERS),ERS group of three kinds of cells were given anhydrous ethanol processing to gain ERS model. Western blot method was adopted to detect the expression level of CHOP and XBP-1 before and after anhydrous ethanol intervention. Results: Before and after ERS,XBP-1 was expressed in three kinds of cells which had not been given anhydrous ethanol processing. Expression level from high to low in turn for LO2 cells,Hep G2 cells,SMMC-7721 cells. After treated with anhydrous ethanol,expression of XBP-1 in three kinds of cells rose; compared with before ERS,of which there was statistically significant difference in the expression of XBP-1 in three kinds of cells between before ERS and after ERS( P < 0. 05). The increase was not statistically significant compared between among the three( P > 0. 05). Before and after ERS,CHOP in Hep G2 cells had no expression. Its expression in LO2 cells is higher,SMMC-7721 cells expression is lower. Compared with before ERS,the expression of CHOP in LO2 and SMMC-7721 cell lysate significantly up-regulated after ERS( P < 0. 05). The alteration of Expression levels varied considerably in SMMC-7721 cells which is more than LO2 cells,it was statistically significant( P < 0. 05). Conclusion: After ERS,CHOP,XBP-1 is associated with malignant cancer of the liver cell differentiation degree,the lower of the degree of differentiation,the greater the expression level changes.

关键词(KeyWords): 肝肿瘤;肝细胞;内质网应激;X-盒-结合蛋白-1;C/EBP同源蛋白
liver carcinoma;hepatocellular;endoplasmic reticulum stress;X box-binding protein-1;C/EBP homologous protein

Abstract:

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基金项目(Foundation): 贵州省科技厅科技项目[黔科合LG(2001)008];; 贵州省教育厅自然科学研究项目[黔教合(2008)022]

作者(Author): 李利波;潘娅;陈腾祥;
LI Libo;PAN Ya;CHEN Tengxiang;Guizhou Medical University;

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