贵州医科大学学报

2017, v.42;No.206(11) 1270-1273

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

人S100A8基因重组慢病毒表达载体的构建
Construction of Recombinant Lentivirus Expressing Vectors of Human S100A8 Gene

杨小燕;金皎;黄璟;许键炜;吴莎莎;马健娟;李燕;庹媛媛;杨红兰;潘海新;胡绍燕;何志旭;
YANG Xiaoyan;JIN Jiao;HUANG Jing;XU Jianwei;WU Shasha;MA Jianjuan;LI Yan;TUO Yuanyuan;YANG Honglan;PAN Haixin;HU Shaoyan;HE Zhixu;Affiliated Hospital of Guizhou Medical University;Experimental Center of Stem Cell and Tissue Engineering,Guizhou Medical University;Children's Hospital Affiliated to Soochow University;

摘要(Abstract):

目的:构建人S100A8基因高表达的重组慢病毒表达载体。方法:以正常人骨髓单个核细胞的c DNA为扩增模板,克隆S100A8基因全长的c DNA,与p MD19-T载体连接后进行测序鉴定;将p LVX-IRES-puro目的载体与测序正确的S100A8片段进行连接,筛选阳性克隆后进行菌落PCR、双酶切及测序鉴定。结果:p LVXS100A8重组质粒双酶切及菌落PCR产物与目的基因相关片段S100A8 c DNA大小一致,成功克隆S100A8基因,S100A8基因成功插入到p LVX-IRES-puro。结论:成功构建了人S100A8基因高表达的重组慢病毒表达载体。
Objective: To construct the recombinant lentiviral expression vector of human S100 A8 gene high expression. Methods: The marrow mononuclear cells c DNA as template in the normal human bone,the full-length c DNA of cloned S100 A8 gene was connected to the p MD19-T vector and identified by sequencing. The p LVX-IRES-puro target vector was connected with the sequenced S100 A8 fragment. After screening the positive clones,colony PCR,double enzyme digestion and sequencing were performed. Results: PLVX-S100 A8 recombinant plasmid double enzyme digestion and colony PCR products have the same size of target gene fragments related to S100 A8 c DNA. The S100 A8 gene was successfully cloned and inserted into p LVX-IRES-puro. Conclusions: The recombinant lentiviral expression vector of human S100 A8 gene high expression is successfully constructed.

关键词(KeyWords): 基因;克隆细胞;高表达;慢病毒载体;构建
gene;clone cell;overexpression;lentiviral vector;construction

Abstract:

Keywords:

基金项目(Foundation): 贵州省科技厅联合基金[黔科合LH(2016)7240号]

作者(Author): 杨小燕;金皎;黄璟;许键炜;吴莎莎;马健娟;李燕;庹媛媛;杨红兰;潘海新;胡绍燕;何志旭;
YANG Xiaoyan;JIN Jiao;HUANG Jing;XU Jianwei;WU Shasha;MA Jianjuan;LI Yan;TUO Yuanyuan;YANG Honglan;PAN Haixin;HU Shaoyan;HE Zhixu;Affiliated Hospital of Guizhou Medical University;Experimental Center of Stem Cell and Tissue Engineering,Guizhou Medical University;Children's Hospital Affiliated to Soochow University;

Email:

DOI: 10.19367/j.cnki.1000-2707.2017.11.007

参考文献(References):

文章评论(Comment):

序号(No.) 时间(Time) 反馈人(User) 邮箱(Email) 标题(Title) 内容(Content)
反馈人(User) 邮箱地址(Email)
反馈标题(Title)
反馈内容(Content)
扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享