贵州医科大学学报

2018, v.43;No.216(09) 1007-1011

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脊髓组织存放时间对冰冻组织切片免疫荧光的影响
Effect of Storage Time of Spinal Cord Tissue on Immunofluorescence of Frozen Tissue Sections

何祥;胡悦;蒋玲;刘树春;
HE Xiang;HU Yue;JIANG Ling;LIU Shuchun;Department of Anesthesiology,Guizhou People's Hospital;Department of Anesthesiology,the First People's Hospital of Shuangliu,Chengdu;

摘要(Abstract):

目的:研究脊髓组织在4%多聚甲醛固定液中的存放时间对冰冻切片抗原NeuN表达的影响。方法:固定于4%多聚甲醛中1年、3月、2月、1月、2周、1周及24 h的脊髓组织,在相同的条件下制作冰冻切片,通过对神经元核抗原(NeuN)的免疫荧光的检测,观察脊髓组织标本在4%多聚甲醛中的固定时间对NeuN表达的影响。结果:在脊髓前角NeuN的表达中,组织固定1月、2月、3月及1年的NeuN阳性率与新鲜标本及组织固定1周、2周比较呈现显著性下降(P <0. 05);在脊髓后角NeuN的表达中,组织固定2月、3月及1年的NeuN阳性率与新鲜标本相比呈现显著性下降(P <0. 05),同时组织固定1年的NeuN阳性率与组织固定1周相比也呈现显著性下降(P <0. 05);在脊髓中央管NeuN的表达中,组织固定3月、1年的NeuN阳性率与新鲜标本及组织固定1周相比呈现显著性降低(P <0. 05),在脊髓组织整体NeuN的表达中,组织固定3月及1年的NeuN阳性率与新鲜标本及组织固定1周、2周比较呈现显著性下降(P <0. 05),同时组织固定2月与新鲜组织及组织固定1周比较呈现显著性下降(P <0. 05)。结论:在同样的环境和条件下,冰冻切片的组织标本越新鲜,其抗原的表达越强。
Objective: To study the effect of storage time of spinal cord tissue in 4% paraformaldehyde fixation solution on the expression of antigens NeuN in frozen sections. Methods: Spinal cord tissues fixed at 4% paraformaldehyde for 1 year,3 months,2 months,1 week and 24 h were made into frozen sections under the same conditions. The effect of fixed time of spinal cord tissue in 4%paraformaldehyde on the expression of( NeuN) was observed by immunofluorescence detection of neuronal nuclear antigen( NeuN). Results: In the expression of NeuN of the anterior horn of spinal cord,the positive rate of NeuN in tissue fixed for 1 month,2 months,3 months and 1 year was significantly lower than that of fresh specimen and tissue fixation for 1 week and 2 weeks( P < 0. 05). In the expression of NeuN of the posterior horn of spinal cord,the positive rate of NeuN in tissue fixed for 2 months,3 months and 1 year was significantly lower than that of fresh specimens( P < 0. 05). At the same time,the positive rate of NeuN in 1 year of tissue fixation was significantly lower than that of 1 week of tissue fixation( P < 0. 05). In the expression of NeuN of the central canal of spinal cord,the positive rate of NeuN in tissue fixed for 3 months and 1 year was significantly lower than that in fresh specimens and tissue fixation for 1 week( P < 0. 05). In the expression of NeuN of spinal cord tissue as a whole,the positive rate of NeuN in tissue fixed for 3 months and 1 year was significantly lower than that in fresh specimen and tissue fixation for 1 week and 2 weeks( P < 0. 05). At the same time,two months of tissue fixation showed a significant decrease compared with fresh tissue and tissue fixation for 1 week( P < 0. 05). Conclusion: Under the same environment and conditions,the fresher the frozen sections are,the stronger the antigen expression is.

关键词(KeyWords): 冰冻切片术;免疫荧光测定;脊髓,组织
frozen section;immunofluorescence;spinal cord,tissue

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基金项目(Foundation): 贵州省卫计委科学技术基金项目(gzwjkj2018-1-018)

作者(Author): 何祥;胡悦;蒋玲;刘树春;
HE Xiang;HU Yue;JIANG Ling;LIU Shuchun;Department of Anesthesiology,Guizhou People's Hospital;Department of Anesthesiology,the First People's Hospital of Shuangliu,Chengdu;

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