贵州医科大学学报

2013, v.38;No.164(05) 489-492

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金莲花药材高效液相色谱指纹图谱研究
HPLC Fingerprint Analysis of Trollius chinensis

谭安菊;周雯;陈慧;沈国华;许祖超;李勇军;兰燕宇;王爱民;
TAN Anju;ZHOU Wen;CHEN Hui;SHEN Guohua;XU Zuchao;LI Yongjun;LAN Yanyu;WANG Aimin;School of pharmacology,Guiyang Medical College;Guizhou Yikang Pharmaceutical Limited Company;

摘要(Abstract):

目的:建立金莲花药材高效液相色谱(HPLC)指纹特征图谱的方法。方法:采用Phenomenex Gemini C18柱(4.6 mm×250 mm,5μm)、乙腈-0.1%磷酸为流动相梯度洗脱,流速1.0 mL/min,柱温35℃,检测波长230 nm,用国家药典委员会颁布的中药色谱指纹图谱相似度评价系统(2004A版)进行相似度评价。结果:建立金莲花药材指纹图谱,确定31个共有峰,指认了5个色谱峰,金莲花各药材指纹图谱与共有模式相似度均在0.85以上。结论:HPLC指纹图谱为金莲花药材质量的更有效控制提供了依据。
Objective: To establish HPLC fingerprint analysis method of Trollius chinensis.Methods: The HPLC method was carried out on Phenomenex Gemini C18(4.6 mm × 250 mm,5μm) using acetonitrile-0.1% phosphoric acid as mobile phase.The flow rate was 1.0 mL / min.The column temperature was 35 ℃.Detection wavelength was 230 nm.The similarity was evaluated with similarity evaluation system for chromatographic fingerprint of tradition Chinese medicine(2004A) issued by state pharmacopoeia committee.Results: HPLC fingerprint of Trollius chinensis was established.31 common peaks were identified in the fingerprint and 5 chromatographic peaks were obtained.The similarity between the mutual mode and each Trollius chinensis fingerprint was more than 0.85.Conclusions: The HPLC fingerprint provides basis for the effective quality control of Trollius chinensis.

关键词(KeyWords): 金莲花;色谱法,高压液相;指纹图谱;相似度
Trollius chinensis;chromatography,high pressure liquid;fingerprint;similarity

Abstract:

Keywords:

基金项目(Foundation): 贵阳市云岩区科技局[2010]第14号;; 贵州省科技厅中药现代化[2011]5081号

作者(Author): 谭安菊;周雯;陈慧;沈国华;许祖超;李勇军;兰燕宇;王爱民;
TAN Anju;ZHOU Wen;CHEN Hui;SHEN Guohua;XU Zuchao;LI Yongjun;LAN Yanyu;WANG Aimin;School of pharmacology,Guiyang Medical College;Guizhou Yikang Pharmaceutical Limited Company;

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DOI: 10.19367/j.cnki.1000-2707.2013.05.012

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