贵州医科大学学报

2013, v.38;No.164(05) 509-511

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两种培养基对卵母细胞体外成熟效果比较
Comparison of the Effects of Two Kinds of Culture Media on Maturation of Human Oocytes in vitro

周桦;何峻;周从容;
ZHOU Hua;HE Jun;ZHOU Congrong;Reproductive Medicine Centre,the Affiliated Hospital of Guiyang Medical College;People's Hospital of Guizhou Province;

摘要(Abstract):

目的:观察两种培养基在未成熟卵母细胞体外培养时,对卵子成熟及授精后胚胎发育的影响。方法:144枚未成熟卵母细胞随机分为两组行体外成熟培养,组1为囊胚培养基,共培养80枚卵子;组2为人输卵管液培养基,共培养64枚卵子培养,两种培养基除基础液不同,其余组分及含量均相同,比较两组卵子的体外成熟、受精及胚胎发育情况。结果:组1卵子成熟率(86.25%)明显高于组2(60.94%),差异有统计学意义(P<0.05);两组卵子的受精率、卵裂率及优胚率无明显差异(P>0.05),但组1有优于组2的趋势。结论:两种培养基均可将未成熟卵母细胞培养成熟,但囊胚培养基更优于人输卵管液培养基,且方便配制,更适用于临床。
Objective: To compare the effects of two kinds of culture media on maturation of human oocytes in vitro,and to observe embryonic development in them.Methods: One hundred and forty four immature human oocytes were randomly divided into two groups and cultured in vitro.The components of the 2 culture media were the same except the foundational medium which was blastocyst medium in group 1(n = 80) and fertilization medium in group 2(n = 64).The maturation rates,fertilization rates,embryonic development situation were compared between the two groups.Results: The maturation rate in group 1(86.25%) was significantly higher than that in group 2(60.94%)(P < 0.05).There was no significant difference in fertilization rate,cleavage rate,and good quality embryo rate between the two groups,but the data of group 1 were better than that of group 2.Conclusions: Both the two kinds of culture media could induce human oocytes to mature in vitro,but blastocyst medium is better than fertilization medium.

关键词(KeyWords): 卵母细胞;体外研究;培养基;胚胎;成熟率;受精率;卵裂率;优胚率
oocytes;in-vitro;culture media;embryo;maturation rate;fertilization rate;cleavage rate;good quality embryo rate

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作者(Author): 周桦;何峻;周从容;
ZHOU Hua;HE Jun;ZHOU Congrong;Reproductive Medicine Centre,the Affiliated Hospital of Guiyang Medical College;People's Hospital of Guizhou Province;

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DOI: 10.19367/j.cnki.1000-2707.2013.05.019

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