贵州医科大学学报

2020, v.45;No.243(12) 1422-1425+1432

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PI3K信号通路的关键信号分子AKT和mTOR在胶质瘤中的表达及意义
Expression of Signaling Molecular AKT and mTOR of PI3K Signaling Pathway in Glioma

吴国敏;史珂蔓;刘娟;冯玲;杨宇石;李珀;徐澍;
WU Guomin;SHI Keman;LIU Juan;FENG Ling;YANG Yushi;LI Po;XU Shu;Department of Oral Histopathology,School of Stomatology,Guizhou Medical University;Department of Pathology,School of Clinical Medicine,Guizhou Medical University;

摘要(Abstract):

目的:探讨人脑胶质瘤组织中磷脂酰肌醇3'-激酶(PI3K)信号通路的关键信号分子激酶B(AKT)和雷帕霉素靶蛋白(m TOR)在人脑胶质瘤中的表达及意义。方法:收集45例胶质瘤患者的临床资料和病理诊断存档蜡块,同时收集同期25例其他非肿瘤病变的脑血管疾病患者的脑组织存档蜡块为对照组;采用免疫组织化学链霉菌抗生物素蛋白—过氧化物酶法(SP法)检测AKT及m TOR蛋白的表达,应用EXCEL、SPSS 25. 0统计软件分析AKT及m TOR蛋白与胶质瘤患者临床病理特征的关系。结果:实验组脑组织细胞AKT及m TOR蛋白阳性表达率均高于对照组,差异有统计学意义(P <0. 05);实验组AKT和m TOR存在显著正相关关系(r=0. 448,P <0. 01),不同性别、年龄、肿瘤发生部位及病理分级与AKT及m TOR蛋白阳性表达率之间比较,差异均无统计学意义(P> 0. 05)。结论:PI3K信号通路的关键信号分子AKT及m TOR在脑胶质瘤组织中存在过表达现象,但该表达与胶质瘤患者的临床病理特征无关。
Objective: To investigate the expression of key signaling molecular kinase B( AKT) and rapamycin target( m TOR) proteins in phosphatidylinositol 3 '-kinase( PI3 K) signaling pathway in human glioma tissues. Methods: Clinical data and pathological diagnosis of 45 patients with glioma were collected. At the same time,25 patients with other non-tumor cerebrovascular diseases were collected as control group. The expression of AKT and m TOR proteins was detected by the( SP)method of immunohistochemical streptomyces biotin-peroxidase. The relationship between AKT and m TOR proteins and clinicopathological features of glioma patients were analyzed by using EXCEL and SPSS 25. 0 statistical software. Results: The expression rates of AKT and m TOR protein in brain tissue cells were higher than those in control group,and the difference was statistically significant( P <0. 05). There was significant positive correlation between AKT and m TOR in experimental group( r =0. 448,P < 0. 01). There was no significant difference in sex, age, tumor location, and the comparison of pathological grade,AKT,and expression rate of m TOR protein positive( P > 0. 05).Conclusion: The key signal molecular AKT and m TOR of PI3 K signaling pathway are overexpressed in glioma tissue, but this expression is independent of clinicopathological characteristics of glioma patients.

关键词(KeyWords): 神经胶质瘤;免疫组织化学;PI3K信号通路;AKT;雷帕霉素靶蛋白
glioma;immunohistochemistry;PI3K signaling pathway;AKT;m TOR

Abstract:

Keywords:

基金项目(Foundation): 贵阳市科技局项目[筑科合(20161001)43];; 贵州省科技厅联合资金项目[黔科合LH字(2016)7241]

作者(Author): 吴国敏;史珂蔓;刘娟;冯玲;杨宇石;李珀;徐澍;
WU Guomin;SHI Keman;LIU Juan;FENG Ling;YANG Yushi;LI Po;XU Shu;Department of Oral Histopathology,School of Stomatology,Guizhou Medical University;Department of Pathology,School of Clinical Medicine,Guizhou Medical University;

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DOI: 10.19367/j.cnki.2096-8388.2020.12.011

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