贵州医科大学学报

2016, v.41;No.190(07) 851-854

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哮喘患者CD4~+ CD25~+调节性T细胞比例与炎症因子的相关性
Correlation between the CD4~+ CD25~+ T Regulatory Cells Proportion and Serum Inflammatory Factors in Peripheral Blood of Asthmatic Patients

高炜;
GAO Wei;Beijing Renhe Hospital;

摘要(Abstract):

目的:探讨哮喘患者外周血CD4~+CD25~+调节性T细胞比例与血清中炎症因子的相关性。方法:110例哮喘患者均分为哮喘急性发作组和哮喘缓解组,55例健康体检者作为对照组;用流式细胞仪测定哮喘患者外周血中CD4~+CD25~+调节性T细胞的比例,酶联免疫法(ELISA)检测被检者血清中白细胞介素18(IL-18)、IL-4及转化生长因子β(TGF-β)含量;用Spearman统计方法分析哮喘患者外周血中CD4~+CD25~+调节性T细胞的比例与血清中IL-18、IL-4和TGF-β含量的相关性。结果:与对照组比较,哮喘缓解组、哮喘急性发作组患者全血细胞中CD4~+CD25~+T细胞比例降低,血清中IL-18、IL-4及TGF-β含量升高(P<0.05);与哮喘缓解组比较,哮喘急性发作组外周血中CD4~+CD25~+T细胞的比例降低而血清中IL-18、IL-4及TGF-β含量升高(P<0.05);Spearman相关分析结果显示,哮喘患者外周血CD4~+CD25~+T细胞比例均与血清IL-18、IL-4和TGF-β含量呈显著负相关关系(r=-0.712、-0.629、-0.593,P=0.006、0.008、0.005)。结论:外周血CD4~+CD25~+T细胞的比例与哮喘的发生和发展密切相关,其机制可能与IL-18、IL-4和TGF-β升高有关。
Objective: To investigate the relationship between the CD4~+CD25~+T regulatory cells proportion and serum inflammatory factors in peripheral blood of asthmatic patients. Methods: 110 cases of patients with asthma were selected as the research subjects,and divided into asthma acute attack group( n = 55) and asthma remission group( n = 55). Another 55 healthy people were selected as control group. Enzyme linked immunosorbent assay( ELISA) was used to detect the content of IL-18,IL-4 and TGF-β,and flow cytometry was used to detect the proportion of CD4~+CD25~+T regulatory cells in peripheral venous blood. Spearman statistical method was adopted to analyze the correlation between the CD4~+CD25~+T regulatory cells proportion and serum inflammatory factors in peripheral blood of asthmatic patients. Results: Compared with control group,CD4~+CD25~+T regulatory cells proportion of blood cell in asthma acute attack group and asthma remission group was significantly decreased while the content of IL-18,IL-4 and TGF-β significantly was increased( P < 0. 05). Compared with asthma remission group,in asthma acute attack group the CD4~+CD25~+T regulatory cells proportion of blood cell was significantly decreased while the content of IL-18,IL-4 and TGF-β significantly was increased( P < 0. 05). The correlation analysis showed that the contents of IL-18,IL-4 and TGF-β in asthma patients were negatively correlated with the proportion of CD4~+CD25~+T regulatory cells( r =-0. 712,- 0. 629,- 0. 593,P = 0. 006,0. 008,0. 005). Conclusions: The proportion of CD4+CD25+regulatory T cells is closely related to the occurrence and development of asthma,and its mechanism may be related to content increase of IL-18,IL-4 and TGF-β.

关键词(KeyWords): 哮喘;白细胞介素类;转化生长因子β;CD4+CD25+调节性T细胞;因素分析,统计学
asthma;interleukins;transforming growth factor β;CD4+ CD25+ T regulatory cells;factors analysis,statistics

Abstract:

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作者(Author): 高炜;
GAO Wei;Beijing Renhe Hospital;

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DOI: 10.19367/j.cnki.1000-2707.2016.07.027

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