贵州医科大学学报

2017, v.42;No.206(11) 1241-1246

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过表达microRNA-137对体外人胰腺癌细胞增殖能力的影响
The Effect of miRNA-137 on Proliferation of Pancreatic Cancer Cell

何志伟;喻超;田舍;陈世裕;张乙凡;潘耀振;孙诚谊;
HE Zhiwei;YU Chao;TIAN She;CHEN Shiyu;ZHANG Yifan;PAN Yaozhen;SUN Chengyi;Department of Hepatobiliary Surgery,the Affiliated Hospital of Guizhou Medical University;Key Laboratory of Liver,Gallbladder,Pancreas and Spleen,Guizhou Medical University;

摘要(Abstract):

目的:过表达microRNA-137(miR-137)对体外人胰腺癌增殖能力的影响及其机制初探。方法:将胰腺癌PANC-1和As PC细胞分为miR-137 mimics转染组和对照组,并通过qRT-RCR检测转染效率;利用流式细胞仪检测细胞凋亡率和周期阻滞变化,CCK-8实验、平板克隆实验检测miR-137对胰腺癌细胞增殖能力的影响,qRT-RCR、Western blot等实验检测miR-137对PTN的mRNA及蛋白表达的影响。结果:上调miR-137表达后,PANC-1、As PC胰腺癌细胞凋亡率增加(P<0.05)、周期阻滞于G1期比例增加(P<0.05);CCK8及平板克隆实验结果均显示过表达miR-137后,胰腺癌细胞增殖能力降低PTN表达显著下调。结论:miR-137在胰腺癌细胞中的表达水平影响抑制胰腺癌的增殖能力,其机制可能与抑制PTN的表达有关。
Objective: To investigate the effect of miR-137 on the proliferation of pancreatic cancer cells and to study the mechanism. Methods: The pancreatic cancer PANC-1 and As PC cells were divided into miR-137 mimics transfection group and control group,and the transfection efficiency was detected by qRT-RCR. Flow cytometry was used to detect the apoptosis rate and the changes of cell cycle arrest,The effect of miR-137 on the proliferation of pancreatic cancer cells was detected by CCK-8 assay and plate cloning assay. The effects of miR-137 on the mRNA and protein expression levels of PTN were measured by PCR and Western blot. Results: miR-137 up-expression effectively inhibited the apoptosis rate and controled cell cycle arrest of pancreatic cancer cell( P < 0. 05). The proliferation ability of PANC-1 and As PC cells decreased significantly after over-expression of miR-137( P < 0. 05).Meanwhile miR-137 over-expression decreased the expression level of PTN( P < 0. 05). Conclusion:miR-137 inhibits the proliferation of pancreatic cancer cells possibly through down-regulating the PTN expression level.

关键词(KeyWords): microRNA-137;胰腺肿瘤;增殖;转染;流式细胞术
microRNA-137;pancreatic cancer;proliferation;transfection;flow cytometry

Abstract:

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基金项目(Foundation): 国家国际科技合作专项资助项目(2014DFA31420);; 国家自然科学基金资助项目(81560477);; 贵州省科学技术厅-贵州医科大学附属医院联合基金[黔科合LH字(2016)7229号];; 贵州省第四批人才基地基金资助[黔省专合字(2012)94号];; 贵州省科学技术厅-贵阳医学院院士工作站肝胆外科分站[黔科合院士站(2015)4013];; 贵州医科大学肝胆胰脾重点实验室项目资助

作者(Author): 何志伟;喻超;田舍;陈世裕;张乙凡;潘耀振;孙诚谊;
HE Zhiwei;YU Chao;TIAN She;CHEN Shiyu;ZHANG Yifan;PAN Yaozhen;SUN Chengyi;Department of Hepatobiliary Surgery,the Affiliated Hospital of Guizhou Medical University;Key Laboratory of Liver,Gallbladder,Pancreas and Spleen,Guizhou Medical University;

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DOI: 10.19367/j.cnki.1000-2707.2017.11.002

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