贵州医科大学学报

2016, v.41;No.190(07) 793-796

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低频电刺激对耐药癫痫大鼠海马细胞外液谷氨酸及γ-氨基丁酸的影响
Influence of Low Frequency Electrical Stimulation on Glutamate and Gama-Aminobutyric Acid in Hippocampal Extracellular Fuid of Rats with Drug Resistant Epilepsy

唐太峰;伍国锋;
TANG Taifeng;WU Guofeng;Department of Neurology,the Second People's Hospital of Guiyang City;Department of Neurology,the Affiliated Hospital of Guizhou Medcial University;

摘要(Abstract):

目的:观察低频电刺激(LFS)海马对杏仁核电点燃耐药癫痫模型大鼠脑内谷氨酸(Glu)及γ-氨基丁酸(GABA)的影响。方法:选取60只雄性健康SD大鼠制作杏仁核点燃模型,采用苯妥英钠(PHT)和苯巴比妥(PB)对杏仁核点燃癫痫鼠进行耐药筛选,对明确耐药大鼠给予海马LFS治疗,收集电刺激海马治疗前后脑组织微透析液,采用高效液相色谱法(HPLC)观察治疗前后Glu及GABA含量。结果:筛选出的耐药癫痫模型大鼠7只,海马刺激前GABA浓度为(29.114 0±7.236 2)mg/L,刺激后为(37.130 0±7.622 5)mg/L,刺激前后比较差异有统计学意义(P<0.05);Glu刺激前浓度为(2 527.742 0±514.831 1)mg/L,刺激后为(2 243.906 0±329.277 8)mg/L,有降低趋势,但差异无统计学意义(P>0.05),GABA/Glu刺激前为0.011 63±0.002 34,刺激后为0.016 50±0.002 36,差异有统计学意义(P<0.05)。结论:耐药癫痫大鼠海马LFS可增加刺激部位脑组织透析液中GABA水平,提高GABA/Glu比值,这可能是LFS抑制癫痫发作的机制之一。
Objective: To observe the influence of low frequency electrical stimulation( LFS) of hippocampus on glutamate( Glu) and Gama-Aminobutyric Acid( GABA) in brain tissues of amygdala kindling epilepsy rat model. Methods: 60 healthy male SD rats were selected to make the amygdala kindling epilepsy model. Amygdala kindling rats were screened by Pheytoin sodium and Phenobarbital for their drug resistance. The rats with clear drug resistance received LFS treatment. Hippocampal brain microdialysate liquid was collected before and after LFS treatment and HPLC was adopted to detect Glu and GABA content. Results: 7 amygdale kindling rats with drug resistance were obtained through screening. Among the 7 selected rats with drug resistance,GABA concentration was( 29. 114 ±7. 236 2) mg / L before LFS compared with( 37. 13 ± 7. 622 5) mg / L after LFS,and the differences were statistically significant( P < 0. 05). Glu concentration was( 2 527. 742 0 ± 514. 831 1) mg / L before LFS compared with( 2 243. 906 0 ± 329. 277 8) mg / L after LFS,with Glu concentration showing a reducing trend but no significant differences( P > 0. 05). However,the ratio of GABA / Glu was0. 011 63 ± 0. 002 34 before LFS compared with 0. 016 50 ± 0. 002 36 after LFS,and the differences were statistically significant( P < 0. 05). Conclusions: LFS on hippocampus can increase the level of GABA and ratio of GABA to Glu,which may be one of mechanisms to suppress the epileptic seizure.

关键词(KeyWords): 癫痫;耐药;电刺激疗法;海马;谷氨酸;γ-氨基丁酸;微透析;色谱法,高压液相
epilepsy;drug resistance;electric stimulation therapy;hippocampus;glutamate;gama-aminobutyric acid;microdialysis;chromatography;HLPC,high pressure liquid

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(81241129/H0913);; 长江学者和创新团队发展计划资助项目(TRT13058)

作者(Author): 唐太峰;伍国锋;
TANG Taifeng;WU Guofeng;Department of Neurology,the Second People's Hospital of Guiyang City;Department of Neurology,the Affiliated Hospital of Guizhou Medcial University;

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DOI: 10.19367/j.cnki.1000-2707.2016.07.012

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