贵州医科大学学报

2020, v.45;No.243(12) 1376-1381

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钙蛋白酶抑制剂对雌激素诱导人乳腺癌细胞上皮-间质转化的影响
The Effect of Calpain Inhibitor on Estrogen-Induced EMT in Breast Cancer Cells

金爱;王宏键;董宇华;王旭东;
JIN Ai;WANG Hongjian;DONG Yuhua;WANG Xudong;Department of Physiology,School of Basic Medicine Sciences,Guizhou Medical University;

摘要(Abstract):

目的:探讨钙蛋白酶(Calpain)对17β-雌二醇(E2)诱导人乳腺癌细胞上皮-间质转化(EMT)的影响。方法:以雌激素受体(ER)阳性乳腺癌细胞MCF-7和ER阴性乳腺癌细胞MDA-MB-231细胞为研究模型,分别用二甲基亚砜(DMSO,对照组)、50 nmol/L E2(E2组)及50 nmol/L E2联合10μmol/L Calpeptin(E2+Calpeptin组)处理;采用划痕实验观察各组MCF-7和MDA-MB-231细胞的迁移能力,采用蛋白印迹法检测各组细胞纤连蛋白(FN)和E-钙黏素(E-cadherin)蛋白表达。结果:与对照组比较,E2组MCF-7和MDA-MB-231细胞在24和48 h的迁移率均增加(P <0. 01),细胞中的FN蛋白在24、48和72 h表达均明显上调、E-cadherin蛋白表达明显下调(P <0. 01);与E2组比较,E2+Calpeptin组MCF-7和MDA-MB-231细胞划痕迁移率明显降低,FN表达明显减少、E-cadherin表达明显增加(P <0. 01)。结论:E2可通过Calpain诱导MCF-7和MDA-MB-231乳腺癌细胞的EMT及细胞迁移。
Objective: To investigate the effect of calpain on epithelial-mesenchymal transition( EMT) induced by 17β-estradiol( E2). Methods: Estrogen receptor( ER) positive breast cancer cell line MCF-7( ER +) and ER negative breast cancer cell MDA-MB-231( ER-) were used as model cells. Cells were divided into control group( DMSO),E2 group( 50 nmol/L),and E2 + Calpeptin group( 50 nmol/L E2 + 10 μmol/L Calpeptin); then cell migration ability of MCF-7 and MDA-MB-231 cells was observed by wound healing assay,and the expression of fibronectin( FN) and Ecadherin( E-cadherin) was detected by Western blot. Results: Compared with the control group,the migration of MCF-7 and MDA-MB-231 cells were increased significantly in 24 h and 48 h( P < 0. 01)and the expression of FN was significantly increased and E-cadherin significantly decreased in E2 group at 24 h,48 h and 72 h( P < 0. 01). Compared with the E2 group,cell migration in E2 + Calpeptin group was significantly decreased( P < 0. 01),while the expression of FN was significantly decreased and E-cadherin was significantly increased( P < 0. 01). Conclusion: E2 can induce EMT and cell migration of MCF-7 and MDA-MB-231 breast cancer cells via calpain.

关键词(KeyWords): 细胞运动;17β-雌二醇;钙蛋白酶;乳腺癌细胞;上皮-间质转化
cell movement;17β-estradiol(E2);calpain;breast cancer cells;epithelial-mesenchymal transition(EMT)

Abstract:

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基金项目(Foundation): 国家自然科学基金资助项目(31660345,31360252)

作者(Author): 金爱;王宏键;董宇华;王旭东;
JIN Ai;WANG Hongjian;DONG Yuhua;WANG Xudong;Department of Physiology,School of Basic Medicine Sciences,Guizhou Medical University;

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DOI: 10.19367/j.cnki.2096-8388.2020.12.003

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