贵州医科大学学报

2013, v.38;No.164(05) 461-464+469

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高表达HIF-1α对肾癌细胞增殖及细胞内STC-1、Ca~(2+)水平的影响
Effects of High Expression of Hypoxia Inducible Factor-1α on Proliferation of Renal Carcinoma Cells,and Stanniocalcin 1 and Ca~(2+) Levels inside Cells

杨清滔;谷江;张永春;杨永安;王楠;朱致晖;祝庆亮;
YANG Qingtao;GU Jiang;ZHANG Yongchun;YANG Yongan;WANG Nan;ZHU Zhihui;ZHU Qingliang;Department of Urinary Surgery,the Affiliated Hospital of Guiyang Medical College;Department of Urinary Surgery,Jiangdu People's Hospital of Yangzhou City;

摘要(Abstract):

目的:研究高表达缺氧诱导因子(HIF-1α)对肾癌(GRC-1)细胞增殖和细胞内斯钙素-1(STC-1)、Ca2+水平的影响。方法:构建HIF-1α重组质粒,转染GRC-1细胞,MTT法检测GRC-1细胞的增殖,RT-PCR法检测GRC-1细胞内HIF-1α及STC-1的表达,荧光探针检测细胞内Ca2+水平,同时设正常GRC-1细胞作为对照组。结果:与对照组相比,转染了HIF-1α的GRC-1细胞增殖率和STC-1表达水平显著增高(P<0.05),Ca2+水平明显降低(P<0.05)。结论:HIF-1α可能参与了肾癌细胞的恶性增殖,其机制可能与STC-1下调肿瘤细胞内Ca2+水平有关。
Objective: To investigate the effect of high expression of hypoxia inducible factor-1α(HIF1α) on renal carcinoma(GRC-1) cell proliferation and the levels of stanniocalcin(STC-1) and Ca2+ inside the cells.Methods: HIF-1α recombinant plasmid was constructed and transfected GRC-1 cells.The GRC-1 proliferation was observed with MTT assay.RT-PCR was used to assess the expression levels of HIF-1α and STC-1.Ca + levels were tested by fluorescence probe.GRC-1 cells that did not received transfection served as control group.Results: Proliferation rates and STC-1 levels in HIF-1α transfected GRC-1 cells were higher and Ca2+ level was lower than those in GRC-1 of control group(P < 0.05).Conclusion: HIF-1α is involved in malignant proliferation of renal carcinoma cells,The mechanism may relate to down-regulating of Ca2+ level in the cell by STC-1.

关键词(KeyWords): 肾肿瘤;癌;细胞;缺氧诱导因子;斯钙素;钙
kidney neoplasms;carcinoma;cells;stanniocalcin;hypoxia inducible factor;calcium

Abstract:

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基金项目(Foundation): 贵州省科技厅社会攻关计划项目(黔科合sy字[2011]3060)

作者(Author): 杨清滔;谷江;张永春;杨永安;王楠;朱致晖;祝庆亮;
YANG Qingtao;GU Jiang;ZHANG Yongchun;YANG Yongan;WANG Nan;ZHU Zhihui;ZHU Qingliang;Department of Urinary Surgery,the Affiliated Hospital of Guiyang Medical College;Department of Urinary Surgery,Jiangdu People's Hospital of Yangzhou City;

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DOI: 10.19367/j.cnki.1000-2707.2013.05.005

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