杨清滔;谷江;张永春;杨永安;王楠;朱致晖;祝庆亮;
YANG Qingtao;GU Jiang;ZHANG Yongchun;YANG Yongan;WANG Nan;ZHU Zhihui;ZHU Qingliang;Department of Urinary Surgery,the Affiliated Hospital of Guiyang Medical College;Department of Urinary Surgery,Jiangdu People's Hospital of Yangzhou City;

• 1:贵阳医学院附院泌尿外科
• 2:扬州市江都人民医院泌尿外科

摘要(Abstract)：

目的:研究高表达缺氧诱导因子(HIF-1α)对肾癌(GRC-1)细胞增殖和细胞内斯钙素-1(STC-1)、Ca2+水平的影响。方法:构建HIF-1α重组质粒,转染GRC-1细胞,MTT法检测GRC-1细胞的增殖,RT-PCR法检测GRC-1细胞内HIF-1α及STC-1的表达,荧光探针检测细胞内Ca2+水平,同时设正常GRC-1细胞作为对照组。结果:与对照组相比,转染了HIF-1α的GRC-1细胞增殖率和STC-1表达水平显著增高(P<0.05),Ca2+水平明显降低(P<0.05)。结论:HIF-1α可能参与了肾癌细胞的恶性增殖,其机制可能与STC-1下调肿瘤细胞内Ca2+水平有关。
Objective: To investigate the effect of high expression of hypoxia inducible factor-1α(HIF1α) on renal carcinoma(GRC-1) cell proliferation and the levels of stanniocalcin(STC-1) and Ca2+ inside the cells.Methods: HIF-1α recombinant plasmid was constructed and transfected GRC-1 cells.The GRC-1 proliferation was observed with MTT assay.RT-PCR was used to assess the expression levels of HIF-1α and STC-1.Ca + levels were tested by fluorescence probe.GRC-1 cells that did not received transfection served as control group.Results: Proliferation rates and STC-1 levels in HIF-1α transfected GRC-1 cells were higher and Ca2+ level was lower than those in GRC-1 of control group(P < 0.05).Conclusion: HIF-1α is involved in malignant proliferation of renal carcinoma cells,The mechanism may relate to down-regulating of Ca2+ level in the cell by STC-1.

关键词(KeyWords)： 肾肿瘤;癌;细胞;缺氧诱导因子;斯钙素;钙
kidney neoplasms;carcinoma;cells;stanniocalcin;hypoxia inducible factor;calcium

Abstract:

Keywords:

基金项目(Foundation): 贵州省科技厅社会攻关计划项目(黔科合sy字[2011]3060)

作者(Author): 杨清滔;谷江;张永春;杨永安;王楠;朱致晖;祝庆亮;
YANG Qingtao;GU Jiang;ZHANG Yongchun;YANG Yongan;WANG Nan;ZHU Zhihui;ZHU Qingliang;Department of Urinary Surgery,the Affiliated Hospital of Guiyang Medical College;Department of Urinary Surgery,Jiangdu People's Hospital of Yangzhou City;

Email:

DOI: 10.19367/j.cnki.1000-2707.2013.05.005

参考文献(References)：

• [1]Vaupel P.Hypoxia and aggressive tumor phenotype:implications for therapy and prognosis[J].Oncologist,2008(3):21-26.
• [2]Bordoli MR,Stiehl DP,Borsig L,et a1.Protyl-4-hydroxylase PHD2 and hypoxia-inducible factor 2-dependent regulation of amphiregulin contributes to breast tumorigenesis[J].Oncogene,2011(5):548-560.
• [3]Bertout JA,Patel SA,Simon MC.The impact of O2availability on human cancer[J].Nat Rev Cancer,2008(12):967-975.
• [4]张延涛,王静蓉,李晓燕.人类斯钙素生物学特性的相关研究[J].医学综述,2009(19):2926-2928.
• [5]郝卫刚,张海蓉,段丽平.人类斯钙素与肿瘤相关性研究进展[J].重庆医学,2011(36):3725-3727.
• [6]Liu G,Yang G,Chang B,et al.Stanniocalcin 1 and ovarian tumorigenesis[J].J Natl Cancer Inst,2010(11):812-827.
• [7]Gort EH,Groot AJ,van der Wall E,et al.Hypoxic regulation of metastasis via hypoxia-inducible factors[J].Curr Mol Med,2008(8):60-67.
• [8]Manolescu B,Oprea E,Busu C,et al.Natural compounds and the hypoxia-inducible factor(HIF)signaling pathway[J].Biochimie,2009(11):1347-1358.
• [9]舒博,申钧,陈鹏,等.HIF-1α和p53蛋白的表达对肾透明细胞癌预后的影响[J].现代泌尿外科杂志,2010(6):435-439.
• [10]Semenza GL.Defining the role of Hypoxia-inducible factor 1 in cancer biology and therapeutics[J].Oncogene,2010(5):625-634.
• [11]Lal A,Peters H,St Croix B,et a1.Transcriptional response to hypoxia in human tumors[J].J Natl Cancer Inst,2001(17):1337-1343.
• [12]Neumann AK,Yang J,Biju MP,et a1.Hypoxia inducible factor 1 alpha regulates T cell receptor signal transduction[J].Proc Natl Acad Sci USA,2005(47):17071-17076.
• [13]He LF,Wang TT,Gao QY,et a1.Stanniocalcin-1 promotes tumor angiogenesis through up-regulation of VEGF in gastric cancer cells[J].J Biomed Sci,2011(39):145-151.
• [14]Loretz CA,Pollina C,Hyodo S,et al.Extracellular calcium sensing receptor distribution in osmoregulatory and endocrine tissues of the tilapia[J].Gen Comp Endocrinology,2009(2):216-228.

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