贵州医科大学学报

2016, v.41;No.192(09) 1002-1005

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microRNA-29c过表达对人胰腺癌AsPC-1、PANC-1细胞增殖的影响
Effect of microRNA-29c Over-expression on Proliferation of Human Pancreatic Cancer Cells

周显飞;田舍;王杰;贾亮亮;喻超;江建新;
ZHOU Xianfei;TIAN She;WANG Jie;JIA Liangliang;YU Chao;JIANG Jianxin;Department of Hepatobiliary Surgery,Affiliated Hospital of Guizhou Medical University;

摘要(Abstract):

目的:探讨microRNA-29c过表达对人胰腺癌As PC-1、PANC-1细胞增殖的影响。方法:构建含microRNA-29c过表达腺病毒并感染至胰腺癌As PC-1及PANC-1细胞(实验组),感染空载体作为阴性对照组,采用实时定量逆转录聚合酶链反应(qRT-PCR)检测As PC-1和PANC-1感染microRNA-29c 24 h时的感染效率,CCK-8实验检测microRNA-29c感染48 h时人胰腺癌细胞的增殖能力,细胞平板克隆实验检测感染microRNA-29c24 h人胰腺癌细胞的单克隆形成能力。结果:与阴性对照组比较,microRNA-29c感染As PC-1及PANC-1细胞后microRNA-29c表达水平明显升高;过表达microRNA-29c后As PC-1和PANC-1细胞增殖能力明显受到抑制,细胞的克隆形成数明显减少(P<0.01)。结论:microRNA-29c过表达能有效抑制胰腺癌细胞的增殖能力,有望成为治疗胰腺癌的新靶标。
Objective: To investigate the effect of miR-29 c over-expression on the proliferation of human pancreatic cancer cells,As PC-1、PANC-1. Methods: The recombination adenovirus Ad-miR-29 c and control adenovirus was constructed and transfected human pancreatic cancer cells,As PC-1 and PANC-1 cell( experiment group). The expressions of miR-29 c in As PC-1 and PANC-1 cells were detected by real-time PCR. The CCK-8 assay was applied to examine the proliferation ability of miR-29 c on the proliferation of human pancreatic cancer cell. The cell colony formation assay was used to measure the growth of the cells after infected by miR-29 c for 24 h. Results: After infected with Ad-miR-29 c and PANC-1,the miR-29 c expression levels increased; the over-expression of microRNA-29 c in the pancreatic cancer cells significantly inhibited the proliferation abilities of As PC-1 and PANC-1cells,cell clone formation abilities were also significantly decreased( P < 0. 01). Conclusion: miR-29 c can effectively suppress the proliferation of human pancreatic cancer cells,which makes a promising new therapeutic target for pancreatic cancer.

关键词(KeyWords): 微小RNA;microRNA-29c;胰腺癌;细胞增殖;腺病毒
micro RNA;miR-29c;pancreatic cancer;cell proliferation;adenovirus

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基金项目(Foundation): 国家国际科技合作专项资助(2014DFA31420);; 国家自然科学基金资助项目(81160311)

作者(Author): 周显飞;田舍;王杰;贾亮亮;喻超;江建新;
ZHOU Xianfei;TIAN She;WANG Jie;JIA Liangliang;YU Chao;JIANG Jianxin;Department of Hepatobiliary Surgery,Affiliated Hospital of Guizhou Medical University;

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