贵州医科大学学报

2016, v.41;No.195(12) 1415-1418

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PCOS雌性模型大鼠血清、卵巢及肾上腺的雄激素水平
Androgen Levels in Female SD Rats with Polycystic Ovarian Syndrome

王厚梅;黄林;肖子文;
WANG Houmei;HUANG Lin;XIAO Ziwen;Department of Obstetrics and Gynecology,the Affiliated Hospital of Guizhou Medical University;

摘要(Abstract):

目的:探讨多囊卵巢综合征(PCOS)雌性SD模型大鼠血清、卵巢及肾上腺的雄激素水平。方法:选取雌性SD大鼠,随机分为对照组和实验组,对照组以基础饲料喂养,自由饮食饮水;实验组进高脂高糖饮食,5%葡萄糖水,第1~42天给予诺和灵N(低精蛋白锌胰岛素NPH-Humulin)股内侧皮下注射,第11~22天同时给予人绒毛膜促性腺激素皮下注射,6周处死大鼠;观察大鼠卵巢组织学及卵巢重量,同时用酶联免疫吸附法(ELISA)法测定血清黄体生成素(LH)、卵泡生成素(FSH)、睾酮(T)、游离睾酮(FT)、硫酸脱氢表雄酮(DHEAS),同时检测卵巢组织及肾上腺组织匀浆T、FT、DHEA-S表达。结果:实验组大鼠卵巢组织镜下见囊性扩张的卵泡增多,黄体及各种发育阶段卵泡减少,对照组卵巢镜下见多个黄体及不同发育阶段的卵泡,卵泡内颗粒细胞呈多层,多为8~9层,造模成功;实验组大鼠卵巢重量、血清T、LH、卵巢组织匀浆T均高于对照组,差异有显著性(P<0.05);实验组大鼠血清FSH、FT、DEAH-S与对照比较差异均无显著性(P>0.05);实验组大鼠卵巢组织匀浆FT、DHEA-S及肾上腺组织匀浆T、FT、DEAH-S与对照比较差异均无显著性(P>0.05)。结论:多囊卵巢综合征雌性SD大鼠模型的高雄激素血症主要表现为高睾酮血症,且卵巢所分泌睾酮影响高雄激素血症发生可能性较大。
Objective:To investigate the androgen levels in serum,ovary and adrenal gland in PCOS SD rats.Methods:Female SD rats were randomly divided into control group and test group.The control group was fed with the basic diet food and uncontrolled water;test group was fed with high-fat and high-sugar diet,5% glucose water,and on 1 to 42 days was given Novolin N(low protamine zinc insulin NPH-Humulin),vastus medialis subcutaneous injection,while on 11 to 22 days was injected with human chorionic gonadotropin,then killed the rats.Observing weight of ovaries and ovarian tissue pathological,then the serum levels of luteinizing hormone(LH),follicle stimulating hormone(FSH),testosterone(T),free-testosterone(FT),dehydroepiandr-Osteronesulfate(DHEA-S) was observed by using ELISA,meanwhile T,FT,DHEA-S levels in ovarian tissue and adrenal tissue were tested.Results:Microscopically,the test group showed the large follicular cystic increased,corpus luteum and follicles at various developmental stage decreased.While the control group showed many corpus luteum and follicles at each development stage,granulosa cells lined with 8-9 layers.These showed the models built successfully.The weight of ovaries,the serum T level,the serum LH level and the ovarian tissue T level of test group were higher than the control group(P<0.05).The results of the serum FSH,FT,DEAH-S comparison between the two groups had no significant difference(P>0.05).The ovarian FT,DHEA-S and the adrenal T,FT,DEAH-S had no significant difference between the test group and the control group(P>0.05);Conclusion:High level serum testosterone is the mainly manifestation of hyperandrogenism,the ovary maybe the major organ to secret it.

关键词(KeyWords): 多囊卵巢综合症;卵巢;肾上腺;血清;大鼠,Sprague-Dawley;高雄激素症
polycystic ovary syndrome;ovary;adrenal gland;serum;rats;Sprague-Dawley;hyperandrogenism

Abstract:

Keywords:

基金项目(Foundation): 贵州省科技厅攻关课题SY[(2011)3021号]

作者(Author): 王厚梅;黄林;肖子文;
WANG Houmei;HUANG Lin;XIAO Ziwen;Department of Obstetrics and Gynecology,the Affiliated Hospital of Guizhou Medical University;

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DOI: 10.19367/j.cnki.1000-2707.2016.12.011

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