贵州医科大学学报

2019, v.44;No.222(03) 306-310

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吗啡对背根神经节FcγR Ⅰ受体介导神经病理痛的作用及机制
Effects of Morphine on Neuropathic Pain Mediated by FcγR Ⅰ Receptor in Dorsal Root Ganglion and Its Mechanism

宋阜彪;梁映霞;类维富;
SONG Fubiao;LIANG Yingxia;LEI Weifu;Department of Anesthesia,Weifang Medical College;Qilu Hospital,Shandong University;

摘要(Abstract):

目的:探讨吗啡对背根神经节FcγRⅠ受体介导神经病理痛的作用及机制。方法:60只成年SD大鼠随机均分为模型组(肠线结扎法制作神经病理痛模型)和吗啡组(建神经病理痛模型后给予吗啡注射液干预1周),比较干预前及干预后第3、7及14天时两组大鼠机械性缩足阈值(MWT)、右后爪热痛潜伏期(TWL),比较干预前及干预后第14天时两组大鼠脊髓组织FcγRⅠmRNA及蛋白表达、背根神经节中生长相关蛋白43(GAP-43)及神经生长因子(NGF)表达。结果:干预前,两组大鼠MWT及TWL数值、脊髓FcγRⅠmRNA及蛋白表达水平、GAP-43及NGF灰度值比较,差异无统计学意义(P> 0. 05);干预后第3、7及14天时吗啡组MWT较模型组同时点显著升高、TWL较同时点模型组显著降低(P <0. 05),干预后第14天时吗啡组FcγRⅠmRNA及蛋白水平较模型组显著降低(P <0. 05)、吗啡组GAP-43、NGF灰度值显著低于模型组,差异有统计学意义(P<0. 05)。结论:SD大鼠神经病理痛与背根神经节FcγRⅠ受体表达水平上调有关,吗啡可作为神经病理痛的有效镇痛药物,其机制可肯能为抑制背根神经节FcγRⅠ受体、下调背神经根神经节内GAP-43及NGF表达有关。
Objective: To investigate the effects of morphine on neuropathic pain induced by Fc-γ receptor type I( FcγR Ⅰ) receptor in dorsal root ganglion and analyze its mechanism. Methods: 60 adult Sprague-Dawley rats were divided into the model group( neuropathic pain models were made by ligation of intestinal thread) and the treatment group( 1 week of intervention with morphine injection was carried out after establishment of neuropathic pain models). The changes of mechanical contraction threshold( MWT) and the right hind paw thermal pain latency( TWL) before the intervention and on the 3 rd,7 th and 14 th day after intervention were compared between the two groups. Changes of the mRNA expression level of FcγR Ⅰ receptor and FcγR Ⅰ receptor protein,the expression of growth-associated protein 43( GAP-43) and nerve growth factor( NGF) in dorsal root ganglion were compared between the two groups before the intervention and on the 14 th day after intervention. Results: There was no significant difference in MWT,TWL,the mRNA expression level of FcγR Ⅰ receptor and FcγR Ⅰ receptor protein,and the gray values of GAP-43 and NGF between the two groups before intervention( P> 0. 05). On the 3 rd,7 th and 14 th day after intervention,the MWT of the treatment group was significantly higher than that of model group,while the TWL in the treatment group was significantly lower than that of model group( P < 0. 05). On the 14 th day after intervention,the mRNA expression level of FcγR Ⅰ receptor and FcγR Ⅰ receptor protein in the treatment group were significantly lower than those of model group.( P < 0. 05). The gray values of GAP-43 and NGF in the treatment group were significantly lower than those ofmodel group after intervention,and the difference was statistically significant( P < 0. 05). Conclusion: Neuropathic pain is closely related to the up-regulation of FcγR Ⅰ receptor in dorsal root ganglion. Morphine can be used as an effective analgesic for neuropathic pain. The possible mechanism may be effective inhibition of the expression of FcγR Ⅰ receptor in dorsal root ganglion and down-regulation of the expression of GAP-43 and NGF in dorsal root ganglia.

关键词(KeyWords): FcγR Ⅰ受体;背根神经节;神经病理痛;吗啡;基因表达;生长相关蛋白43;神经生长因子
FcγR Ⅰreceptor;dorsal root ganglion;neuropathic pain;morphine;gene expression;growth associated protein;nerve growth factor

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基金项目(Foundation): 山东省自然科学基金资助项目(ZR2012HL27)

作者(Author): 宋阜彪;梁映霞;类维富;
SONG Fubiao;LIANG Yingxia;LEI Weifu;Department of Anesthesia,Weifang Medical College;Qilu Hospital,Shandong University;

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