贵州医科大学学报

2018, v.43;No.208(01) 16-20

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类风湿汤对类风湿性关节炎大鼠血清RF、抗-CCP抗体的影响
Effect of "Rheumatoid Soup" on Serum RF and Anti-CCP Antibody Levels of Rats with Rheumatoid Arthritis

黄少炎;杨守盘;罗先意;史红美;王光义;吕明庄;
HUANG Shaoyan;YANG Shoupan;LUO Xianyi;SHI Hongmei;WANG Guangyi;LV Mingzhuang;Guizhou Medical University;Department of Acupuncture and Moxibustion,Affiliated Hospital of Guizhou Medical University;

摘要(Abstract):

目的:观察中药"类风湿汤"对类风湿性关节炎(RA)大鼠血清RF、抗-CCP抗体的影响。方法:40只SD大鼠均分为对照组、模型组、中药组和阳性对照组;大鼠左后足跖底部皮下注射0.15 m L弗氏完全佐剂1次复制RA模型、对照组注射0.15 m L生理盐水,造模后中药组大鼠按18.9 g/kg灌胃"类风湿汤"3 m L(1次/d),对照组、模型组灌胃等量生理盐水(1次/d),阳性对照组给予2.0 mg/kg甲氨蝶呤MTX灌胃(1次/周),共治疗21 d;于造模前和治疗21 d时,观察4组大鼠的体毛色泽、神志、四肢活动、关节肿胀情况及饮食等一般情况,于造模后和治疗21 d时,采用容积法测量4组大鼠左后足肿胀度;于治疗结束时,取各组大鼠,采用血清ELASA法检测RF和抗-CCP含量,取大鼠跖趾关节滑膜组织观察病理变化。结果:造模前各组大鼠一般情况良好,关节无肿胀;造模后,模型组大鼠一般情况比对照组差,足趾关节肿胀度高于对照组(P<0.05);治疗后,与模型组比较,中药组大鼠一般情况有所改善,关节肿胀度减轻差异有统计学意义(P<0.05),但中药组与阳性比较,差异无统计学意义(P>0.05);治疗后,与对照组比较,模型组大鼠抗-CCP以及RF含量升高(P<0.05),但中药组与阳性比较,差异无统计学意义(P>0.05);与模型组比较,中药组、西药组大鼠血清抗-CCP以及RF含量均降低(P<0.05),但中药组与阳性比较,差异无统计学意义(P>0.05);治疗后,与模型组比较,中药组和西药组滑膜细胞增生减轻,水肿明显减轻,血管增生和浸润细胞数量减少。结论:"类风湿汤"对AA大鼠的治疗作用可能与降低大鼠血清抗-CCP抗体以及RF含量有关。
Objective: To Effect of Chinese medicine " Rheumatoid Soup" on serum RF and antiCCP antibody levels of rats with rheumatoid arthritis( RA). Methods: Forty SD rats were divided into control group,model group,Chinese medicine group,western medicine group. 0. 15 m L Freund complete adjuvant was subcutaneously injected into the bottom of the left hind foot of rats in the model group,Chinese medicine group and western medicine group to replicate RA model,and control group was given 0. 15 m L physiological saline instead. After modeling,the rats in the Chinese medicine group were gavaged with 3 m L 18. 9 g/kg " rheumatoid soup"( 1 times/d),the control group and the model group were given the same amount of normal saline( 1 times/d),and the western medicine group was treated with 2 mg/kg MTX( 1 times/week). The treatment time was 21 d. The body hair color,spirit,extremity movement,joint swelling and diet were observed in the 4 groups of rats before modeling and after treatment for 21 d. The swelling degree of left hind feet of 4 groups of rats was measured by volume method after modeling and after treatment for 21 d. At the end of the treatment,ELASA method was used to detect the serum content of RF and anti-CCP,and the pathological changes of the synovial membrane of the metatarsophalangeal joint of rats were observed. Results: Before modeling,the rats in each group were generally in good condition and the joints were not swollen. After modeling,the general condition of the model group was worse than that of the control group,and the swelling degree of the toe joints was higher than that of the control group( P < 0. 05). After the treatment,compared with model group,the general situation of Chinese medicine group and western medicine group was improved,and the difference of the joint swelling degree was statistically significant( P< 0. 05). After treatment,compared with the control group,the anti-CCP and RF contents in the model group increased( P < 0. 05). Compared with the model group,the levels of anti-CCP and RF in the serum of the Chinese medicine group and Western medicine group were all decreased( P < 0. 05).After treatment,compared model group,the synoviocyte proliferation of Chinese medicine group and western medicine group alleviated,the edema decreased obviously,the blood vessel proliferation and the number of infiltrating cells decreased. Conclusion: The therapeutic effect of " rheumatoid soup" on RA rats may be related to the reduction of serum anti-CCP antibody and RF content in rats.

关键词(KeyWords): 类风湿性关节炎;类风湿汤;类风湿因子;抗-CCP抗体
rheumatoid arthritis;rheumatoid soup;rheumatoid factor;anti-CCP antibody

Abstract:

Keywords:

基金项目(Foundation): 国家中医药管理局全国名老中医药专家传承工作室建设项目(2014)

作者(Author): 黄少炎;杨守盘;罗先意;史红美;王光义;吕明庄;
HUANG Shaoyan;YANG Shoupan;LUO Xianyi;SHI Hongmei;WANG Guangyi;LV Mingzhuang;Guizhou Medical University;Department of Acupuncture and Moxibustion,Affiliated Hospital of Guizhou Medical University;

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DOI: 10.19367/j.cnki.1000-2707.2018.01.004

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