贵州医科大学学报

2019, v.44;No.224(05) 519-523+535

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贵州黔南喀斯特洞穴放线菌抗菌活性筛选及差异研究
Isolation and Identification of Actinomycetes with Antibacterial Activity from Karst Caves in Southern Guizhou

牛雪可;兰永哲;李文均;曹煜;黄劲;廖万清;康颖倩;
NIU Xueke;LAN Yongzhe;LI Wenjun;CAO Yu;HUANG Jing;LIAO Wanqing;KANG Yingqian;Department of Microbiology, School of Basic Medical Sciences, Guizhou Medical University;Guizhou Talent Base Program of Guizhou Provincial Government,Key Laboratory of Medical Microbiology and Parasitology of Education Department of Guizhou,Guizhou Medical University;School of Life Sciences,Sun Yat-sen University;Department of Dermatology and Venereal Disease,the Affiliated Hospital of Guizhou Medical University;Institu

摘要(Abstract):

目的:探索贵州黔南荔波喀斯特地区洞穴放线菌资源,筛选具有抗菌活性的放线菌菌株。方法:采集贵州荔波茂兰喀斯特森林无名洞穴内土壤样品,采用纯培养的方法分离菌株,并扩增其16S rRNA基因、筛选其中放线菌;使用3种培养基发酵,管碟法对放线菌进行抗菌活性检测。结果:共分离出35株放线菌,分属于3个目、5科、9个属、22个种,红球菌属为优势菌属(45.7%);42.8%的菌株对至少一种测试菌具有抗菌活性;使用AM3及ISP5培养基获得具有抗性发酵液的比例均占53.3%,RA培养基只有33.3%的发酵液具有抗性。结论:贵州黔南喀斯特无名洞穴内存在丰富的具备潜在抗菌活性的放线菌资源,不同的发酵培养条件影响放线菌抗菌活性。
Objective: To explore local actinobacteria resources, and screen actinobacteria strains with antibacterial activity in Southern Guizhou China. Methods: Soil samples from unknown caves in Maolan forest were collected and isolated by pure culture. The 16S rRNA gene was amplified and the actinomycetes were screened. Five kinds of culture media were used for fermentation, and the antimicrobial activity of three clinical pathogens was detected by cylinder platr method. Results: A total of 35 actinomycetes from Karst caves were isolated, belonging to 3 orders, 5 families, 9 genera and 22 species. The antimicrobial activity of these actinomycetes were tested aganist pathogenic bacteria. The result suggested that 42.8% of these strains showed antimicrobial activity against at least one tested bacterium. The media used for the growth played a crucial role in the antimicrobial activity. The rate of the antimicrobial activity was highest in AM3 and ISP5(55.3%) followed by R2A(33.3%). Conclusion: There are abundant actinobacteria with antimicrobial activity in Karst caves. Fermentation media have influence on the antimicrobial activity.

关键词(KeyWords): 喀斯特;放线菌;抗菌活性;培养条件;筛选;贵州
Karst;actinomycetes;antimicrobial activity;culture conditions;screen;Guizhou

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基金项目(Foundation): 贵州省科技厅优秀青年科技人才培养计划项目[黔科合(20175639)];贵州省科技厅科技支撑计划项目[黔科合支撑(2019)2873];; 贵州省卫计委医疗卫生援黔专家团廖万清院士工作室项目[gzwjkj2016-1-050];; 贵阳市科技局项目[筑科合同(2017)5-19]

作者(Author): 牛雪可;兰永哲;李文均;曹煜;黄劲;廖万清;康颖倩;
NIU Xueke;LAN Yongzhe;LI Wenjun;CAO Yu;HUANG Jing;LIAO Wanqing;KANG Yingqian;Department of Microbiology, School of Basic Medical Sciences, Guizhou Medical University;Guizhou Talent Base Program of Guizhou Provincial Government,Key Laboratory of Medical Microbiology and Parasitology of Education Department of Guizhou,Guizhou Medical University;School of Life Sciences,Sun Yat-sen University;Department of Dermatology and Venereal Disease,the Affiliated Hospital of Guizhou Medical University;Institu

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DOI: 10.19367/j.cnki.1000-2707.2019.05.005

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