贵州医科大学学报

2012, v.37;No.158(05) 486-489

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维生素D_3联合地塞米松诱导大鼠破骨细胞的体外培养
Induction of the Formation of Rat Osteoclast with the Combination of Vitamin D_3 and Dexamethasone in Vitro

董强;夏茜;马洪;王永;
DONG Qiang1,XIA Qian2,MA Hong3,WANG Yong1(1.Department of Prosthodontics,the Affiliated Hospital of Guiyang Medical College,Guiyang 550004,Guizhou, China;2.Department of Oral Medicine,the Affiliated Hospital of Guiyang Medical College,Guiyang 550004, Guizhou,China;3.Department of Oral and Maxillofacial Surgery,the Affiliated Hospital of Guiyang Medical College,Guiyang 550004,Guizhou,China)

摘要(Abstract):

目的:观察VitD3联合地塞米松对大鼠骨髓体外培养破骨细胞的影响,探索提高破骨细胞体外培养效果的有效途径。方法:实验分为两组,以维生素D3骨髓诱导法为对照组,以维生素D3联合地塞米松诱导SD大鼠骨髓细胞为实验组,通过Trap染色、甲苯胺蓝染色和扫描电镜观察鉴定破骨细胞,并对计数结果进行统计学分析。结果:实验组与对照组均获得Trap染色阳性及在体外有噬骨能力的破骨细胞,实验组破骨细胞计数(培养第7天)多于对照组,有统计学差异(P<0.05)。结论:与维生素D3单独诱导相比,维生素D3联合地塞米松体外诱导大鼠破骨细胞的效率更高。
Objective: To establish a method for inducing rat bone marrow cells into osteoclasts in vitro,and to explore the effective way to improve the effect of culturing osteoclasts in vitro.Methods: Vitamin D3 bone marrow cell induction method served as control.Vitamin D3 combined with dexamethasone were used to induce Sprague-Dawley rat bone marrow cells into osteoclasts.The osteoclasts were identified by Trap staining,toluidine blue staining and scanning electron microscope(SEM) observation.The count results of osteoclasts were analyzed statistically.Results: Trap-positive osteoclasts which had the ability of bone absorption were obtained in the two groups.Statistical differences were found in the amounts of osteoclasts between the two groups(P<0.05).Conclusion: Compared with inducing by vitamin D3 alone,inducing the formation of rat osteoclasts by vitamin D3 combined with dexamethasone have better effect.

关键词(KeyWords): 破骨细胞;细胞,培养的;维生素D3;地塞米松
osteoclasts;cell,cultured;vitamin D3;dexamethasone

Abstract:

Keywords:

基金项目(Foundation): 贵州省优秀科技教育人才省长专项基金(黔省专合字2007-117号);; 贵州省科学技术基金【黔科合J字(2009)2308号】

作者(Author): 董强;夏茜;马洪;王永;
DONG Qiang1,XIA Qian2,MA Hong3,WANG Yong1(1.Department of Prosthodontics,the Affiliated Hospital of Guiyang Medical College,Guiyang 550004,Guizhou, China;2.Department of Oral Medicine,the Affiliated Hospital of Guiyang Medical College,Guiyang 550004, Guizhou,China;3.Department of Oral and Maxillofacial Surgery,the Affiliated Hospital of Guiyang Medical College,Guiyang 550004,Guizhou,China)

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DOI: 10.19367/j.cnki.1000-2707.2012.05.009

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