贵州医科大学学报

2009, v.34;No.140(05) 508-511

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缺血性急性肾功能衰竭大鼠血清TNF-α水平及肾小管上皮细胞Fas、Bcl-2的表达
Serum TNF-α Level in Acute Renal Failure Rat and Expression of Fas and Bcl-2 in Its Renal Tubular Epithelia Cells

姜军;陈珊琳;王予川;
JIANG Jun1,CHEN Shanlin2,WANG Yuchuan2(1. Department of Pediatrics,Guizhou Provincial People's Hospital,Guiyang 550002,Guizhou,China;2. Department of Pediatrics,The Affiliated Hospital of Guiyang Medical College,Guiyang 550004,Guizhou,China)

摘要(Abstract):

目的:探讨缺血性急性肾功能衰竭(IARF)大鼠血清TNF-α水平、肾小管上皮细胞中Fas和Bcl-2的表达及细胞凋亡情况,分析它们在IARF发病机制中的作用。方法:用无损伤动脉夹夹闭大鼠双侧肾蒂40min制作IARF模型,观察缺血再灌注(IR)后2、6、12、24和48h时血清TNF-α及肌酐(SCr)水平,观察各时间点肾脏组织的病理学变化,观察IR12h时肾小管上皮细胞Fas、Bcl-2表达及细胞凋亡。结果:IR12h时肾小管上皮细胞凋亡明显,以皮髓交界处为著,阳性细胞率增加,P<0.01;IR12h时,血清TNF-α和SCr明显增多,P<0.01;IR12h时肾小管上皮细胞Fas表达增加(P<0.01),Bcl-2表达减少(P<0.01);IR各各时间点肾组织均有不同程度的细胞破坏,以IR6h和12h最严重。结论:IR所致的IARF动物模型中存在细胞凋亡的病理改变,严重影响肾功能;血清TNF-α可能通过上凋Fas和下调Bcl-2影响肾小管上皮细胞的凋亡过程。
Objective:To investigate serum TNF-α content,apoptosis,and expression of Fas and Bcl-2 in renal tubular epithelia cells (RTEC)of ischemic acute renal failure (IARF) rat,and to analyze their effects in IARF pathogenesy. Methods: SD rat model of IARF was set up by occluding of bilateral renal pedicle for 40 min and following reperfusion. Serum creatine (SCr) and TNF-α,and kidney tissue pathological changes were observed at time points of 2h,6h,12h,24h,and 48h after ischemia-reperfusion (IR).At 12h after IR,the expression of Fas,and Bcl-2 in renal tubular epithelia cells and cell apoptosis were investigated. Result: In 12h after IR,cell apoptosis was obvious,specially at the juncture of cortex and medulla showing increased positive rate (P<0.01).Serum SCr and TNF-α significantly raised (P<0.01),and in RTEC,Fas expression increased (P<0.01) while that of Bcl-2 deceased (P<0.01);Different degrees of cell destroy were found at different time points,and it's most serious at 6 h and 12 h. Conclusion: The pathological changes of cell apoptosis occur in IR caused IARF animal model and influence renal function severely;Serum TNF-α might affect apoptosis process in RTEC by up-regulate Fas and down-regulate Bcl-2.

关键词(KeyWords): 肾功能衰竭,急性;缺血;肿瘤坏死因子;Fas;Bcl-2;大鼠,Sprague-dawley
kidney failure,acute;ischemia;tumor necrosis factor;fas;Bcl-2;rats,sprague-dawley

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作者(Author): 姜军;陈珊琳;王予川;
JIANG Jun1,CHEN Shanlin2,WANG Yuchuan2(1. Department of Pediatrics,Guizhou Provincial People's Hospital,Guiyang 550002,Guizhou,China;2. Department of Pediatrics,The Affiliated Hospital of Guiyang Medical College,Guiyang 550004,Guizhou,China)

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DOI: 10.19367/j.cnki.1000-2707.2009.05.009

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