贵州医科大学学报

2006, (01) 49-50+53

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高效液相色谱法测定妇科止带胶囊中盐酸小檗碱的含量
The Determination of Berberine Hydrochloride in Fukezhidai Capsules with HPLC

何迅;李勇军;王爱民;刘丽娜;兰燕宇;王永林;
HE Xun,LI Yong-jun,WANG Ai-min,LIU Li-na,LAN Yan-yu,WANG Yong-lin(School of Pharmacy,Guiyang Medical College,Guiyang 550004,China)

摘要(Abstract):

目的:探讨妇科止带胶囊中盐酸小檗碱的含量测定方法。方法:采用高效液相色谱法测定盐酸小檗碱的含量。色谱柱D iamonsil C18柱,流动相为甲醇-0.7%三乙胺溶液(磷酸调节pH值至3)50∶50,流速1 m l/m in,检测波长265 nm。结果:该方法线性关系良好,平均加样回收率为100.5%(n=9),RSD=1.8%。结论:本方法简便、测定结果准确、重现性好,可控制妇科止带胶囊的质量。
Objective: To establish a method for the determination of the berberine hydrochloride contents in Fukezhidai capsules.Methods: The HPLC with a C_(18) column was used.The mobile phase was methanol-0.7% triethylamine(adjusted to pH 3 with H_3PO_4)(50∶50) with a flow rate of 1 ml/min,and the UV detection wavelength was 265 nm.Results: There was a good linear correlation.The average recovery rate was 100.5%(n=9),RSD=1.8%.Conclusion: This method is simple,accurate,reliable and could be used for quality control of Fukezhidai capsule.

关键词(KeyWords): 植物制剂;小檗碱;色谱法,高压液相;盐酸小檗碱;妇科止带胶囊
plant preparation;berberine;chromatography,high pressure liquid;berberine hydrochloride;Fukezhidai capsule

Abstract:

Keywords:

基金项目(Foundation): 贵州省中药现代化科技产业资助项目[黔科合中药专字(2005)5042号]

作者(Author): 何迅;李勇军;王爱民;刘丽娜;兰燕宇;王永林;
HE Xun,LI Yong-jun,WANG Ai-min,LIU Li-na,LAN Yan-yu,WANG Yong-lin(School of Pharmacy,Guiyang Medical College,Guiyang 550004,China)

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DOI: 10.19367/j.cnki.1000-2707.2006.01.015

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