韦锦文,陈绍林
WEI Jin-wen, CHEN Shao-lin (The Second People’s Hospital of Guizhou Province, Guiyang 550004, China)

• 1:贵州省第二人民医院
• 2:贵州省第二人民医院 贵州贵阳550004
• 3:贵州贵阳550004

摘要(Abstract)：

目的:建立反相高效液相色谱法测定血浆中阿普唑仑浓度的方法。方法:以乙腈 50mmol/L磷酸 盐缓冲液pH=6.0(35∶65V/V)为流动相,三唑仑作内标,在紫外波长220nm处检测。结果:血浆中阿普唑仑的 最低检出浓度为2μg/L,平均回收率为90.82%,线性范围10～200μg/L(r=0.9998)。日内和日间差异(RSD) 分别小于3%和4%。结论:反相高效液色谱法灵敏、快速、准确,可满足临床治疗监测和药代动力学的研究。
Objective: To develop reverse HPLC procedure for the determination of alprazolam in human plasma. Methods: The mobile phase consisted of 50 mmol/L potassium phosphate (pH6.0)-acetonitrile (65∶ 35,V/V) and UV detection wavelength was set at 220nm. Results: The lowest detection limitation of alprazolam was 2μg/L, and the mean recovery rate 90.82%. The linearity was 10 to 200μg/L (r=0.9998) and the relative standard deviations of within-day and between-day were less than 3% and 4% respectively. Conclusion: The method is rapid, sensitive and accurate enough for clinical therapeutic drug monitoring.

关键词(KeyWords)： 阿普唑仑;色谱法,高压液相;药物监测;药代动力学
alprazolam; chromatography,high pressure liquid; drug monitoring; pharmacokinetics

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 韦锦文,陈绍林
WEI Jin-wen, CHEN Shao-lin (The Second People’s Hospital of Guizhou Province, Guiyang 550004, China)

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DOI: 10.19367/j.cnki.1000-2707.2005.01.012

参考文献(References)：

• [1]MazharM,BinderSR.Analysisofbenzodiazepinesandtri cyclicantidepressantsinserumusingacommonsolid phaseclean upandaCommonmobileplase[J].Jchrom atogr,1989,497:201.
• [2]陈绍林,韦锦文.HPLC测定血浆中三唑仑浓度[J].中国药学杂志,1995,30(增刊):133.

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