陈静宇;林新瑜;王芳;林鸿刚;
CHEN Jingyu1,LIN Xinyu2,WANG Fang2,LIN Honggang3(1.Department of Dermatology,the Sixth People's Hospital of Chengdu City,Chengdu 610051,Sichuan,China;2 Institute of Dermatology and Venereology,Sichuan Academy of Medical Sciences & People's Hospital of Sichuan Province,Chengdu 610072,Sichuan,China;3 State Key Laboratory of Biotherapy,Sichuan University,Chengdu 610041,Sichuan,China)

• 1:成都市第六人民医院皮肤科
• 2:四川省医学科学院四川省人民医院皮肤病性病研究所
• 3:四川大学国家生物治疗重点实验室

摘要(Abstract)：

目的:建立正常人表皮黑素细胞体外培养体系,观察不同浓度白介素-8对体外培养正常人黑素细胞的细胞增殖及黑素合成的影响。方法:采用四甲基偶氮唑盐(MTT)法测定不同浓度白介素-8对黑素细胞增值的影响,NaOH裂解法测定黑素生成量,流式细胞仪检测黑素细胞的凋亡率。结果:白介素-8对黑素细胞活力有增强作用,能使细胞增殖能力增加,黑素合成增加,但对黑素细胞的凋亡率无显著性影响。结论:白介素-8对体外培养的黑素细胞增殖及黑素生成都有增强作用。
Objective: To establish the culture system of human normal melanocytes in vitro,and to study the effects of interleukin-8 at different concentrations on the proliferation of human melanocytes and melanin synthesis.Methods: MTT method and NaOH lysis method were employed to measure the effects of interleukin-8 at different concentrations on the proliferation of human melanocytes and melanin respectively.Apoptosis rates of melanocytes were detected with flow cytometer.Results: Interleukin-8 promotes melanocyte proliferation and melanin synthesis.Conclusions: Interleukin-8 enhances melanocyte proliferation and melanin synthesis.

关键词(KeyWords)： 白细胞介素-8;黑素细胞;细胞培养
interleukin-8;melanocytes;cell culture

Abstract:

Keywords:

基金项目(Foundation): 四川省卫生厅资助课题[110219]

作者(Author): 陈静宇;林新瑜;王芳;林鸿刚;
CHEN Jingyu1,LIN Xinyu2,WANG Fang2,LIN Honggang3(1.Department of Dermatology,the Sixth People's Hospital of Chengdu City,Chengdu 610051,Sichuan,China;2 Institute of Dermatology and Venereology,Sichuan Academy of Medical Sciences & People's Hospital of Sichuan Province,Chengdu 610072,Sichuan,China;3 State Key Laboratory of Biotherapy,Sichuan University,Chengdu 610041,Sichuan,China)

Email:

DOI: 10.19367/j.cnki.1000-2707.2012.05.013

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