贵州医科大学学报

2004, (02) 121-125

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富硒蘑菇对小鼠精子运动能力的影响
A Study on Effect of Selenium-enriched Mushroom on the Mobility of Mouse Sperm

庄惠君,卢素琳,彭小峰,夏艳,张催
ZHUANG Hui-jun 1, LU Su-lin 2, PENG Xiao-feng 1, XIA Yan 3, ZHANG Cui 2 (1. Department of Endocrinology, The Affiliated Hospital of Guiyang Medical College, Guiyang 550004, China; 2. Department of Pharmacology, Guiyang Medical College, Guiyang 550004, China; 3. The Affiliated Hospital of Guizhou Institute of Traditional Chinese Medicine, Guiyang 550001, China)

摘要(Abstract):

目的 :研究富硒蘑菇 (SEM )对低硒雄性小鼠精子活力的影响 ,并阐明其作用机理。方法 :用低硒饲料复制低硒小鼠模型 ,灌胃补充富硒蘑菇 ,按灌胃剂量分为低剂量 (SEML)、中剂量 (SEMM)、高剂量 (SEMH) 3组 ,及低硒组 (Se de)和亚硒酸钠组 (Se) ,饲以低硒饲料或亚硒酸钠 112 μg·kg-1·d-1灌胃 ;正常组饲以标准小鼠饲料 ,蒸馏水灌胃。实验历时 4 5d。用计算机辅助精子质量分析 (CASA)技术测定小鼠精子运动速度和轨迹 ,判定精子运动能力 ,用荧光分析法测定睾丸硒谷胱甘肽过氧化物酶 (GSH px)、超氧化物歧化酶 (SOD)活性及丙二醛 (MDA)含量。结果 :SEM各组和Se组精子活力和活率加强 ,A、B级精子百分率增加 ,D级精子减少 ,各项运动参数VCL、VSL、STR、LIN和WOB增加 ,睾丸硒含量增加 ,GSH px和SOD活性显著增强 ,MDA含量降低。比较补充相同含硒量的SEMM组和Se组 ,前者各项指标有略好于后者的倾向。结论 :SEM有抗氧化损伤 ,改善低硒小鼠精子运动能力的作用。
Objective: To study the effect of selenium enriched mushroom (SEM) on the sperm mobility of mice and its antioxidative damage mechanism. Methods: The male kunming mice were used for the experiment, and were divided into the group of low dosage SEM (140 mg·kg -1 ·d -1 ) (SEML), medium dosage (280 mg·kg -1 ·d -1 ) (SEMM), high dosage (560 mg·kg -1 ·d -1 ) (SEMH), selenium deficient group (Se-de), selenium group (Se), and normal control (NC). The Se-de group was fed with selenium scarcity food, and the Se group was given with sodium nitrite at a dosage of 112 μg·kg -1 ·d -1 . NC group was fed on standard diet. SEM or sodium nitrite were continuously given by oral injection for 45 days, except that for the groups Se-de and NC distilled water were injected instead. The computer assisted sperm analysis (CASA) was used for evaluating of sperm mobility and viability. The testis selenium and malonie aldehyde (MDA) content, glutathione peroxidase (GSH-px) and superoxide dismutas (SOD) activity were determined using fluoromettic method, and Chemiluminescence enzyme immunoassay respectively. Results: Of all the mice in groups of SEM and Se, the percentage of grade A and B sperm increased and the grade D decreased. The sperm viability and mobility trace parameters, the sperm viability and the mobile preferences, the average curvilineal velocity (VCL), the average beeline velocity (VSL), straightness (STR), linearity (LIN), and wobble (WOB) increased. The selenium content in the testis increased, but the testis index didn’t change obviously. The activity of GSH-px, SOD in testis increased and the content of MDA decreasesd significantly. The same pharmological effect also showed in mice group Se. As comparing the data of group Se with those of SEMM group that received same amount of selenium, a trend that the effect is enhanced in SEMM group was observed. Conclusion: The results suggest that selenium in SEM could increase the sperm motility and viability, elevate the activity of GSH-px and SOD, and decrease the MDA content of selenium deficient mice.

关键词(KeyWords): 精子;硒;谷胱甘肽过氧化酶;超氧化物歧化酶;丙二醛;小鼠
spermatozoa; Selenium; superoxide dismutase; glutathione peroxidase; malondialdehyde; mice

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作者(Author): 庄惠君,卢素琳,彭小峰,夏艳,张催
ZHUANG Hui-jun 1, LU Su-lin 2, PENG Xiao-feng 1, XIA Yan 3, ZHANG Cui 2 (1. Department of Endocrinology, The Affiliated Hospital of Guiyang Medical College, Guiyang 550004, China; 2. Department of Pharmacology, Guiyang Medical College, Guiyang 550004, China; 3. The Affiliated Hospital of Guizhou Institute of Traditional Chinese Medicine, Guiyang 550001, China)

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DOI: 10.19367/j.cnki.1000-2707.2004.02.012

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