贵州医科大学学报

2006, (02) 131-134

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巨噬细胞集落刺激因子及受体在感染性心内膜炎早期的表达
Roles of Macrophage Colony-stimulating Factor and Its Receptor in the Early Phase of Infective Endocarditis

赵波;付向宁;孙威;李军;潘铁成;
ZHAO Bo,FU Xiang-ning,SUN Wei,LI Jun,PAN Tie-cheng (Department of Thoracardiosurgery,Tongji Hospital,Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430030,China)

摘要(Abstract):

目的:探讨巨噬细胞集落刺激因子及其受体(MCSF-1,c-fm s)在感染性心内膜炎(IE)中的作用和其对瓣膜组织在赘生物形成中的影响。方法:比较研究IE早期家兔心脏瓣膜MCSF-1和c-fm smRNA表达水平变化。7只正常家兔做对照;7只家兔静脉注射5×106CFU金黄色葡萄球菌(金葡菌);7只家兔行二尖瓣手术;7只家兔行二尖瓣手术加静脉注射金葡菌;7只家兔行三尖瓣手术;7只家兔行三尖瓣手术加静脉注射金葡菌。6 h后取出心脏瓣膜,应用逆转录聚合酶链反应(RT-PCR)技术测量MCSF-1和c-fm smRNA表达水平。结果:二尖瓣和三尖瓣MCSF-1 mRNA在各自的房室瓣手术后明显提高表达(P<0.05)。附加金葡菌注射后,二尖瓣MCSF-1 mRNA表达水平进一步提高(P<0.01),而三尖瓣没有明显变化;二尖瓣和三尖瓣c-fm smRNA在各自的房室瓣手术后明显降低表达水平(P<0.05)。结论:M-CSF,c-fm s是参与IE发生的重要因子之一;二尖瓣和三尖瓣在IE早期有不同的MCSF-1,c-fm s表达反应。瓣膜组织的MCSF-1 mRNA表达水平与IE炎症发展状态相平行。
Objective: To explore the roles of macrophage colony-stimulating factor-1(MCSF-1),and its receptor,c-fms,in the development of bacterial endocardial vegetations and to analyze their effects on valve tissue during bacterial colonization and vegetation growth.Methods: Seven rabbits were given 5×10~6 colony forming units of Staphylococcus aureus(i.v.);seven rabbits underwent mitral surgery;seven rabbits underwent tricuspid surgery;seven rabbits underwent mitral surgery and received S.aureus administration;and seven rabbits underwent tricuspid surgery plus S.aureus administration.The hearts were explanted in 6 hours after the intervention and the valvular tissues were shock frozen in liquid nitrogen.Total RNA was isolated and mRNA abundance determined by semiquantitative duplex RT-PCR using 18S RNA as internal control.Results were compared to the control group consisting of seven untreated rabbits(CG).Results: The MCSF-1mRNA expression on atrioventricular valves was significantly increased after corresponding isolated AV-valve surgery.Moreover,in the mitral valve but not in the tricuspid valve,a significant further increase of MCSF-1 mRNA expression was observed accompanying additional bacteremia(P<0.01 compared to isolated surgery).The mRNA of c-fms in atrioventricular valves was significantly decreased after corresponding isolated AV-valve surgery(P<0.05).Conclusions: MCSF-1 and c-fms expression respond differently to mitral and tricuspid valve surgeries in the early phase and bacteremia.The expression level of MCSF-1 mRNA in heart valves could reflect the inflammatory status of infective endocarditis.

关键词(KeyWords): 心内膜炎,细菌性;葡萄球菌,金黄色;巨噬细胞集落刺激因子;基因,c-fms;逆转录聚合酶链反应
endocarditis,bacterial;Staphylococcus aureus;macrophage colony-stimulating factor;gene,c-fms;reverse transcriptase polymerase chain reaction

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基金项目(Foundation): 同济医院科研基金资助(No.2096699486)

作者(Author): 赵波;付向宁;孙威;李军;潘铁成;
ZHAO Bo,FU Xiang-ning,SUN Wei,LI Jun,PAN Tie-cheng (Department of Thoracardiosurgery,Tongji Hospital,Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430030,China)

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