贵州医科大学学报

2005, (01) 15-17

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明胶海绵与人胚半月板细胞构建复合物的研究
A Study on Construction Complex of Human Fetal Meniscus Cells and Gelatin Sponge

叶川,尹培荣,吴承龙,马敏先,宁旭
YE Chuan 1, YIN Pei-rong 1, WU Cheng-long 2, MA Min-xian 3, NIN Xu 1 (1.Department of Orthopaedics Surgery, The Affiliated Hospital of Guiyang Medical College, Guiyang 550004, China; 2.Department of Microbiology, Guiyang Medical College, Guiyang 550004, China; 3.Department of Stomatology, The Affiliated Hospital of Guiyang Medical College, Guiyang 550004, China)

摘要(Abstract):

目的:研究明胶海绵作为半月板组织工程支架材料应用的的可行性。方法:采用胶原酶阶段消化 法获取人胚半月板纤维软骨细胞,取经胰岛素样生长因子I(IGF Ⅰ)扩增的第3代细胞与已用多聚赖氨酸修饰 的明胶海绵复合培养,进行MTT、光镜、扫描电镜观察。结果:纤维软骨细胞于明胶海绵上牢固粘附,4d开始增 殖,3周时已有大量细胞生长于明胶海绵上。结论:明胶海绵表现出良好的亲水性、细胞吸附性和体外生物相容 性,适于作为组织工程细胞培养支架材料用。
Objective: To investigate the possibility of gelatin sponge as fibrocartilage cell scaffold in meniscus tissue-engineering. Methods: Human fetal meniscal fibrochondrocytes were collected with collagenase digestion method. The 3th passage cells proliferated with IGF-I were co-cultured with gelatin-sponge that modified by poly-Lysine and were observed with MTT, light microscop and scanning electronic microscopy. Results: Fibrocartilage cells attached to gelatin sponge closely and begin to proliferate in 4 days. lots of cells were found to proliferate onto gelatin sponge in 3 weeks. Conclusions: Gelatin sponge has good hydrophilia, adsorptivity, biocompatibility with fibrocartilage cells. It can be used as scaffold for meniscus tissue engineering.

关键词(KeyWords): 半月板,胫骨;软骨;细胞;明胶海绵,吸收性;组织工程
menisci,tibial; cartilage; cells; gelatin sponge,absorbable; tissue engineering

Abstract:

Keywords:

基金项目(Foundation): 贵州省卫生厅资助项目(编号G2004 17)

作者(Author): 叶川,尹培荣,吴承龙,马敏先,宁旭
YE Chuan 1, YIN Pei-rong 1, WU Cheng-long 2, MA Min-xian 3, NIN Xu 1 (1.Department of Orthopaedics Surgery, The Affiliated Hospital of Guiyang Medical College, Guiyang 550004, China; 2.Department of Microbiology, Guiyang Medical College, Guiyang 550004, China; 3.Department of Stomatology, The Affiliated Hospital of Guiyang Medical College, Guiyang 550004, China)

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DOI: 10.19367/j.cnki.1000-2707.2005.01.004

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