贵州医科大学学报

2004, (02) 143-144

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高效液相色谱法测定肤痔清软膏中盐酸小檗碱含量
The Determination of Berberine Hydrochloride in Fuzhiqing Ointments by HPLC

陈万里,史蕙,李勇军,王永林
CHEN Wan-li 1, SHI Hui 1, LI Yong-jun 2, WANG Yong-lin 2 (1. Guizhou Provincial Institute for Drug Control, Guiyang 550004, China; 2. School of Pharmacy, Guiyang Medical College, Guiyang 550004, China)

摘要(Abstract):

目的 :建立肤痔清软膏中盐酸小檗碱含量的测定方法。方法 :采用C18柱 ,乙腈 -四氢呋喃 - 0 0 5mol/L磷酸二氢钾 (2 8∶12∶16 4 )为流动相 (0 7%三乙胺 ,磷酸调pH 3) ,流速 1ml/min ,温度 30℃ ,检测波长 35 0nm。结果 :盐酸小檗碱在 0 0 6 16~ 0 30 80 μg之间呈良好的线性关系 ,r =0 9999,平均回收率为 99 0 % ,RSD为2 10 %。结论 :本法简便、准确、重复性好 ,可作为该制剂的定量分析方法。
Objective: To establish a method to determine the contents of berberine hydrochloride in Fuzhiqing ointments. Methods: C 18 column, with acetonitrile-tetrahydrofuran 0.05 mol/l KH 2PO 4 (0.7% triethylamine plus H 3PO 4 adjusted to pH 3 ) (28∶12∶164) was used as mobile phase, and the detection wavelength was 350 nm with a flowing rate of 1 ml/min.The temperature of column was 30 C°. Results: The linear range was 0.061 6 μg~0.308 μg for berberine hydrochloride (r= 0.999 9 ). The average recovery rate was 99.0% and RSD was 2.10%. Conclusion: The method is simple, and accurate with a good reproducibility and can be used for quantitative analysis of this preparation.

关键词(KeyWords): 肤痔清软膏;盐酸小檗碱;色谱法,高压液相
Fuzhiqing ointments; berberine hydrochloride; chromatography,high pressure liquid

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作者(Author): 陈万里,史蕙,李勇军,王永林
CHEN Wan-li 1, SHI Hui 1, LI Yong-jun 2, WANG Yong-lin 2 (1. Guizhou Provincial Institute for Drug Control, Guiyang 550004, China; 2. School of Pharmacy, Guiyang Medical College, Guiyang 550004, China)

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DOI: 10.19367/j.cnki.1000-2707.2004.02.019

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