贵州医科大学学报

2006, (02) 148-150

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反相高效液相色谱法测定三多消袋泡剂中齐墩果酸的含量
Determination of Oleanolic Acid in Sanduoxiao Tea Bag with RP-HPLC

赵平;李勇军;何迅;刘丽娜;
ZHAO ping,LI Yong-jun,HE Xun,LIU Li-na(School of Pharmacology,Guiyang Medical College,Guiyang 550004,China)

摘要(Abstract):

目的:建立三多消袋泡剂中齐墩果酸的含量测定方法。方法:采用反相高效液相色谱法(RP-HPLC)测定齐墩果酸的含量。色谱柱:Krom asil C18(5.0 nm×200μm,5μm);流动相:甲醇-0.5%醋酸溶液(84∶16);流速:1 m l/m in;温度:35℃;检测波长为210 nm。结果:齐墩果酸的线性范围为:0.552~8.832μg(r=0.999 8),平均回收率为99.64,RSD为2.22%(n=5)。结论:反相高效液相色谱法准确、简便、快速、灵敏度高、重现性好,具有实用性,可用于三多消袋泡剂的质量控制。
Objective: To establish a RP-HPLC method for determination of the content of oleanolic acid in Sanduoxiao tea bag.Methods: Kromasil column(5.0 mm×200 mm,5 μm) was used.The mobile phase was methanol-0.2% HAC(84∶16),flow-rate 1.0 ml/min,and the UV detection wavelength 210 nm.Results: The calibration curve was linear in the range of 0.552~8.832 μg(r=(0.999 8)) for oleanolic acid.The average recovery rate was 99.64% with a RSD of 2.22%(n=5).Conclusions: This method is accurate,rapid,sensitive and well repeatable,and is useful for the quality control of Sanduoxiao tea bag.

关键词(KeyWords): 植物制剂;糖尿病,非胰岛素依赖型;色谱法,高压液相;齐墩果酸;三多消袋泡剂
plant preparations;diabetes mellitus,non-insulin-dependent;chromatography,high pressure liquid;oleanolic acid;Sanduoxiao tea bag

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作者(Author): 赵平;李勇军;何迅;刘丽娜;
ZHAO ping,LI Yong-jun,HE Xun,LIU Li-na(School of Pharmacology,Guiyang Medical College,Guiyang 550004,China)

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