贵州医科大学学报

2006, (01) 6-9

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转化生长因子β1基因转染对结肠癌SW480细胞增殖的抑制作用
Suppression Effects of TGF-β1 Gene Infection on Colon Cancer SW480 Cell Growth in vitro

刘嘉;葛海燕;肖卫东;陈祖林;
LIU Jia~1,GE Hai-yan~2,XIAO Wei-dong~1,CHEN Zu-lin~1(1.Department of General Surgery Xinqiao Hospital,The Third Military Medical University,Chongqing 400037,China; 2.Department of General Surgery The Tenth People's Hospital of Shanghai,Shanghai 200072,China)

摘要(Abstract):

目的:探讨转化生长因子β1(TGF-β1)基因转染对结肠癌细胞SW 480体外增殖的抑制作用。方法:以腺病毒为载体将人TGF-β1基因导入体外培养的人结肠癌细胞株SW 480,应用免疫组织化学及逆转录聚合酶链反应(RT-PCR)检测SW 480细胞中转化生长因子β1(TGF-β1)蛋白及mRNA的表达情况。通过MTT法检测转染后细胞增殖的抑制情况。结果:转染hTGF-β1基因后,与对照组相比,TGF-β1蛋白和mRNA在细胞中的表达均增强。SW 480的增殖受到明显抑制(P<0.05),抑制率为60%~80%。结论:转染hTGF-β1基因对体外培养的结肠癌细胞SW 480的增殖有明显抑制作用。
Objective: To investigate the effect of infection with adenoviral vector containing human TGF-β1 cDNA on the proliferation of colon carcinoma SW480 cells in vitro.Methods: SW480 cells were infected with recombinant adenovirus containing TGF-β1 in vitro.Protein and mRNA of TGF-β1 were detected by immunohistochemical method and reverse transcriptase-polymerase chain reaction(RT-PCR) technique respectively.The proliferation of SW480 cells was detected by MTT assay.Results: There was increased expression of TGF-β1 in SW480 cell line with transgenosis treatment at protein and mRNA levels.The ability of cell proliferation decreased significantly in SW480 cell line infected with Ad-hTGF-β1,compared with the control group(P<0.05).The inhibition rates arrived at 60%~80%.Conclusion: The proliferation of the colon carcinoma SW480 cell line may be inhibited by transgenosis of TGF-β1 significantly.

关键词(KeyWords): 结肠肿瘤;转化生长因子β;逆转录聚合酶链反应;免疫组织化学;增殖抑制
colonic neoplasms;transforming growth factor beta;reverse transcriptase polymerase chain reaction;immunohistochemistry;proliferation inhibition

Abstract:

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基金项目(Foundation): 国家自然科学基金资助项目(30271481)

作者(Author): 刘嘉;葛海燕;肖卫东;陈祖林;
LIU Jia~1,GE Hai-yan~2,XIAO Wei-dong~1,CHEN Zu-lin~1(1.Department of General Surgery Xinqiao Hospital,The Third Military Medical University,Chongqing 400037,China; 2.Department of General Surgery The Tenth People's Hospital of Shanghai,Shanghai 200072,China)

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DOI: 10.19367/j.cnki.1000-2707.2006.01.003

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