贵州医科大学学报

2006, (02) 109-112

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小鼠胚胎干细胞培养条件的探索
Culture and Biological Characteristics of Mouse Embryonic Stem Cell

黄璟;何志旭;
HUANG Jing,HE Zhi-xu (Department of Pediatrics,The Affiliated Hospital of Guiyang Medical College,Guiyang 550004,China)

摘要(Abstract):

目的:探讨小鼠胚胎干细胞(ES细胞)的培养特点及生物学特性,为ES细胞的应用研究奠定基础。方法:小鼠胚胎成纤维细胞原代培养,3~5代的细胞作为饲养层细胞培养ES细胞。另在无饲养层细胞、明胶包被培养皿、培养液中含白血病抑制因子条件下培养小鼠ES细胞,观察2种情况下ES细胞的生长情况。结果:小鼠胚胎成纤维细胞呈放射状或旋涡状走行,ES细胞在2种条件下均能呈克隆状生长,且保持未分化状态,碱性磷酸酶(AKP)染色呈阳性。结论:2种条件下ES细胞均能良好生长。
Objective: To investigate the culture and biological characteristics of mouse embryonic stem(ES) cell as a foundational work for its application.Methods: Mouse ES cells were observed in two culture systems: 1) on feeder cells of the primary mouse embryo fibroblasts and 2) in leukemia with inhibititory factor but without feeder cells.Results: The primary mouse embryo fibroblast growth trended radially or circinately.The ES cell clones under the two conditions appeared typical characteristics and maintained un-differentiation state.Conclusion: The ES cells can grow well under those conditions.

关键词(KeyWords): 干细胞;胚胎;细胞,培养的;小鼠,近交BALBC;胚胎干细胞
stem cells;embryo;cells cultured;mice,inbred BALB C;embryonic stem cell

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基金项目(Foundation): 贵州省卫生厅资助项目

作者(Author): 黄璟;何志旭;
HUANG Jing,HE Zhi-xu (Department of Pediatrics,The Affiliated Hospital of Guiyang Medical College,Guiyang 550004,China)

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